Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry.
dc.contributor.author | Masoodi, Mojgan | * |
dc.contributor.author | Mir, Adnan A. | * |
dc.contributor.author | Petasis, N.A. | * |
dc.contributor.author | Serhan, S.N. | * |
dc.contributor.author | Nicolaou, Anna | * |
dc.date.accessioned | 2010-12-15T16:03:09Z | |
dc.date.available | 2010-12-15T16:03:09Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Masoodi, M., Mir, A. A., Petasis, N. A., Serhan, C. N. and Nicolaou, A. (2008). Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry, Vol. 22, No 2., pp.75¿83. | en |
dc.identifier.uri | http://hdl.handle.net/10454/4582 | |
dc.description | no | en |
dc.description.abstract | Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) and exhibit a range of tissue and cell-specific activities in many physiological and pathological processes. Electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/¿L, whilst the limits of detection and quantitation were 10-20 and 20-50 pg respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically-related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxy-eicosapentaenoic and hydroxy-docosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease. | en |
dc.description.sponsorship | Wellcome Trust, British Heart Foundation | en |
dc.language.iso | en | en |
dc.publisher | Wiley | en |
dc.subject | Hydroxy fatty acids | en |
dc.subject | Eicosanoids | en |
dc.subject | Arachidonic Acid | en |
dc.subject | Eicosapentaenoic Acid | en |
dc.subject | Resolvins | en |
dc.subject | Protectin | en |
dc.subject | Docosahexaenoic acid | en |
dc.subject | Polyunsaturated fatty acids (PUFA) | en |
dc.subject | Liquid Chromatograpy Electrospray Ionisation Tandem Mass Spectrometry (LC/ESI-MS/MS) | en |
dc.subject | Lipid mediators | en |
dc.title | Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry. | en |
dc.status.refereed | yes | en |
dc.type | Article | en |
dc.identifier.JournalTitle | Rapid Communications in Mass Spectrometry | en |
dc.type.version | Accepted Manuscript | en |
dc.identifier.doi | https://doi.org/10.1002/rcm.3331 |