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dc.contributor.authorMasoodi, Mojgan*
dc.contributor.authorMir, Adnan A.*
dc.contributor.authorPetasis, N.A.*
dc.contributor.authorSerhan, S.N.*
dc.contributor.authorNicolaou, Anna*
dc.date.accessioned2010-12-15T16:03:09Z
dc.date.available2010-12-15T16:03:09Z
dc.date.issued2008
dc.identifier.citationMasoodi, M., Mir, A. A., Petasis, N. A., Serhan, C. N. and Nicolaou, A. (2008). Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry, Vol. 22, No 2., pp.75¿83.en
dc.identifier.urihttp://hdl.handle.net/10454/4582
dc.descriptionnoen
dc.description.abstractBioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) and exhibit a range of tissue and cell-specific activities in many physiological and pathological processes. Electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/¿L, whilst the limits of detection and quantitation were 10-20 and 20-50 pg respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically-related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxy-eicosapentaenoic and hydroxy-docosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease.en
dc.description.sponsorshipWellcome Trust, British Heart Foundationen
dc.language.isoenen
dc.publisherWileyen
dc.relation.isreferencedbyhttp://dx.doi.org/10.1002/rcm.3331en
dc.subjectHydroxy fatty acidsen
dc.subjectEicosanoidsen
dc.subjectArachidonic Aciden
dc.subjectEicosapentaenoic Aciden
dc.subjectResolvinsen
dc.subjectProtectinen
dc.subjectDocosahexaenoic aciden
dc.subjectPolyunsaturated fatty acids (PUFA)en
dc.subjectLiquid Chromatograpy Electrospray Ionisation Tandem Mass Spectrometry (LC/ESI-MS/MS)en
dc.subjectLipid mediatorsen
dc.titleSimultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray tandem mass spectrometry.en
dc.status.refereedyesen
dc.typeArticleen
dc.identifier.JournalTitleRapid Communications in Mass Spectrometryen
dc.type.versionAccepted Manuscripten


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