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    Detergent addition to trypsin digest and Ion Mobility Separation prior to MS/MS improves peptide yield and Protein Identification for in situ Proteomic Investigation of Frozen and FFPE Adenocarcinoma tissue sections.

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    Publication date
    2009
    Author
    Djidja, M-C.
    Francese, S.
    Loadman, Paul M.
    Sutton, Chris W.
    Scriven, P.
    Claude, E.
    Snel, M.F.
    Franck, J.
    Salzet, M.
    Clench, M.R.
    Keyword
    Ion Mobility
    Adenocarcinoma
    Formalin fixed paraffin embedded
    Imaging
    MALDI
    MCF7
    Stress proteins
    Cancer investigation
    Tumour xenograft
    Protein identification
    Peer-Reviewed
    yes
    
    Metadata
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    Abstract
    The identification of proteins involved in tumour progression or which permit enhanced or novel therapeutic targeting is essential for cancer research. Direct MALDI analysis of tissue sections is rapidly demonstrating its potential for protein imaging and profiling in the investigation of a range of disease states including cancer. MALDI-mass spectrometry imaging (MALDI-MSI) has been used here for direct visualisation and in situ characterisation of proteins in breast tumour tissue section samples. Frozen MCF7 breast tumour xenograft and human formalin-fixed paraffin-embedded breast cancer tissue sections were used. An improved protocol for on-tissue trypsin digestion is described incorporating the use of a detergent, which increases the yield of tryptic peptides for both fresh frozen and formalin-fixed paraffin-embedded tumour tissue sections. A novel approach combining MALDI-MSI and ion mobility separation MALDI-tandem mass spectrometry imaging for improving the detection of low-abundance proteins that are difficult to detect by direct MALDI-MSI analysis is described. In situ protein identification was carried out directly from the tissue section by MALDI-MSI. Numerous protein signals were detected and some proteins including histone H3, H4 and Grp75 that were abundant in the tumour region were identified
    URI
    http://hdl.handle.net/10454/4565
    Citation
    Djidja, M.-C., Francese, S., Loadman, P. M., Sutton, C. W., Scriven, P., Claude, E., Snel, M. F., Franck, J., Salzet, M. and Clench, M. R. (2009). Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin-fixed paraffin-embedded adenocarcinoma tissue sections. Proteomics, Vol. 9, No. 10, pp. 2750¿2763.
    Link to publisher’s version
    http://dx.doi.org/10.1002/pmic.200800624
    Type
    Article
    Collections
    Life Sciences Publications

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