Show simple item record

dc.contributor.authorGill, Jason H.*
dc.contributor.authorKirwan, Ian G.*
dc.contributor.authorSeargent, Jill M.*
dc.contributor.authorMartin, Sandie W.*
dc.contributor.authorTijani, S.*
dc.contributor.authorAnikin, V.A.*
dc.contributor.authorMearns, A.J.*
dc.contributor.authorBibby, Michael C.*
dc.contributor.authorAnthoney, Alan*
dc.contributor.authorLoadman, Paul*
dc.date.accessioned2009-12-17T10:42:15Z
dc.date.available2009-12-17T10:42:15Z
dc.date.issued2004
dc.identifier.citationGill JH, Kirwan IG, Seargent JM et al (2004) MMP-10 is overexpressed, proteolytically active and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia. 6(6): 777-785.
dc.identifier.urihttp://hdl.handle.net/10454/4121
dc.descriptionNo
dc.description.abstractMatrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a major factor for tumor development and expansion. This study analysed MMP-10 protein expression and activity in human lung tumors of various grade, stage, and type to address the relationship between MMP-10 and tumor characteristics and to evaluate MMP-10 as a therapeutic target in non small cell lung carcinoma (NSCLC). Unlike the majority of MMPs, MMP-10 was located in the tumor mass as opposed to tumor stroma. MMP-10 protein was observed at low levels in normal human lung tissues and at significantly higher levels in all types of NSCLC. No correlation was observed between MMP-10 protein expression and tumor type, stage, or lymph node invasion. To discriminate between active and inactive forms of MMP-10 in samples of human NSCLC, we have developed an ex vivo fluorescent assay. Measurable MMP-10 activity was detected in 42 of 50 specimens of lung cancer and only 2 of 10 specimens of histologically normal lung tissue. No relationship was observed between MMP-10 activity levels and clinicopathologic characteristics. Our results suggest that MMP-10 is expressed and active at high levels in human NSCLC compared to normal lung tissues, and, as such, is a potential target for the development of novel therapeutics for lung cancer treatment.
dc.language.isoen
dc.subjectTumour Markers
dc.subjectHeterologous
dc.subjectTransplantation
dc.subjectAnimals
dc.subjectCarcinoma
dc.subjectCell line
dc.subjectTumour
dc.subjectEnzyme activation
dc.subjectImmunohistochemistry
dc.subjectLung neoplasms
dc.subjectHumans
dc.subjectMatrix Metalloproteinase 10
dc.subjectMetalloendopeptidases
dc.subjectMice
dc.subjectBiological analysis
dc.titleMMP-10 is overexpressed, proteolytically active and a potential target for therapeutic intervention in human lung carcinomas
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1593/neo.04283
dc.openaccess.statusclosedAccess


This item appears in the following Collection(s)

Show simple item record