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dc.contributor.authorSmith, Annie G.*
dc.contributor.authorPowis, Rachel A.*
dc.contributor.authorPritchard, D.I.*
dc.contributor.authorBritland, Stephen T.*
dc.date.accessioned2009-12-08T10:36:36Z
dc.date.available2009-12-08T10:36:36Z
dc.date.issued2008
dc.identifier.citationSmith, A. G., Powis, R. A., Pritchard, D. I. and Britland, S, T. (2008). Biotechnology Progress, Vol. 22, No. 6, pp. 1690 - 1696.en
dc.identifier.urihttp://hdl.handle.net/10454/4041
dc.descriptionnoen
dc.description.abstractThe resurgence of larval biotherapy as a debridement tool in wound management has been accompanied by several clinical reports highlighting concomitant tissue regeneration. Studies employing in vitro cell motility assays have found that purified excretory/secretory (ES) products from Greenbottle larvae (blowfly, Lucilia sericata) are motogenic for human dermal fibroblasts when used as a supplement in culture media. The objective of the present study was to determine whether ES delivered using a prototype hydrogel wound dressing induced similar motogenic effects on fibroblastic (3T3) and epithelial cells (HaCaTs) comprising a scratched-monolayer wound model. Quantitative analysis by MTT assay failed to detect significant mitogenic effects of ES on either cell type. Quantitative image analysis revealed that ES exposure markedly accelerated wound closure through a motogenic effect on both fibroblasts and keratinocytes. Quantitative histochemical analysis detected significantly higher phosphotyrosine (pTyr) expression in ES-exposed cell cultures than in controls; moreover immunocytochemistry revealed conspicuously raised levels of pTyr expression in cells located at the wound margin. By attenuation with a panel of enzyme inhibitors these effects were attributed to the protease components of ES. The present results suggest that controlled delivery of ES as a follow-up to maggot debridement therapy may be an effective therapeutic option for stimulation of tissue regeneration in wound management.en
dc.language.isoenen
dc.publisherAmerican Institute of Chemical Engineers (AIChE)en
dc.subjectLarval biotherapyen
dc.subjectDebridementen
dc.subjectWound managementen
dc.subjectTissue regenerationen
dc.subjectGreenbottle larvae (blowfly, Lucilia sericata)en
dc.subjectHydrogel wound dressingen
dc.subjectMaggot debridement therapyen
dc.titleGreenbottle (Lucilia Sericata) larval secretions delivered from a prototype hydrogel wound dressing accelerate the closure of model wounds.en
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionpublished version paperen
dc.identifier.doihttps://doi.org/10.1021/bp0601600


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