Fragmentation of N-linked glycans with a matrix-assisted laser desorption/ionization ion trap time-of-flight mass spectrometer.
Publication date
2004Peer-Reviewed
YesOpen Access status
closedAccess
Metadata
Show full item recordAbstract
N-Linked glycans were ionized from several matrices with a Shimadzu-Biotech AXIMA-QIT matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer. [M+Na]+ ions were produced from all matrices and were accompanied by varying amounts of in-source fragmentation products. The least fragmentation was produced by 2,5-dihydroxybenzoic acid and the most by -cyano-4-hydroxycinnamic acid and 6-aza-2-thiothymine. Sialic acid loss was extensive but could be prevented by formation of methyl esters. Fragmentation produced typical low-energy-type spectra dominated by ions formed by glycosidic cleavages. MSn spectra (n = 3 and 4) were used to probe the pathways leading to the major diagnostic ions. Thus, for example, an ion that was formed by loss of the core GlcNAc residues and the 3-antenna was confirmed as being formed by a B/Y rather than a C/Z mechanism. The proposed structures of several cross-ring cleavage ions were confirmed and it was shown that MS3 spectra could be obtained from as little as 10 fmol of glycanVersion
No full-text in the repositoryCitation
Harvey, D.J., Martin R.L., Jackson, K.A., Sutton, C.W. (2004). Rapid Commununications in Mass Spectrometry. Vol. 18, No. 24, pp. 2997-3007.Link to Version of Record
https://doi.org/10.1002/rcm.1709Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1002/rcm.1709