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dc.contributor.authorUrquhart, Paula*
dc.contributor.authorParkin, Susan M.*
dc.contributor.authorNicolaou, Anna*
dc.date.accessioned2009-12-07T16:13:04Z
dc.date.available2009-12-07T16:13:04Z
dc.date.issued2009-12-07T16:13:04Z
dc.identifier.citationUrquhart, P., Parkin, S. M. and Nicolaou, A. (2001). Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids. Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 65, No. 1, pp. 15-22.en
dc.identifier.urihttp://hdl.handle.net/10454/4038
dc.descriptionnoen
dc.description.abstractHuman saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the14C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and the effect of the n-3 polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid on them. Results indicate that HSVECs while under resting conditions produce mainly prostaglandin F2 ¿(PGF2 ¿). After stimulation with calcium ionophore A23187, the cells were found to synthesise PGI2, PGE2and PGF2¿as major products and thromboxane B2and PGD2as minor products. Production of14C-labelled hydroxyeicosatetraenoic acids was not detected. Eicosapentaenoic acid was found to inhibit basal and stimulated prostanoid production whereas docosahexaenoic acid inhibited basal but strongly increased stimulated prostanoid production. These results may offer the basis for further studies aiming to investigate targets for pharmacological intervention in inflammatory conditions.en
dc.language.isoenen
dc.publisherHarcourten
dc.relation.isreferencedbyhttp://dx.doi.org/10.1054/plef.2001.0282en
dc.subjectHuman saphenous veinen
dc.subjectEndothelial cellsen
dc.subjectEndothelial metabolismen
dc.subjectN-3 polyunsaturated fatty acidsen
dc.subjectDietary fatty acidsen
dc.subjectEicosapentaenoic aciden
dc.subjectDocosahexaenoic aciden
dc.subjectProstaglandin F2 ¿(PGF2 ¿).en
dc.subjectThromboxaneen
dc.subjectCalciumen
dc.titleProfile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acidsen
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionpublished version paperen


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