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dc.contributor.authorBehrendt, M.*
dc.contributor.authorPartridge, L.J.*
dc.contributor.authorGriffiths, B*
dc.contributor.authorGoodfield, M.*
dc.contributor.authorSnaith, M.*
dc.contributor.authorLindsey, Nigel J.*
dc.date.accessioned2009-12-03T15:44:05Z
dc.date.available2009-12-03T15:44:05Z
dc.date.issued2003
dc.identifier.citationBehrendt, M., Partridge, L. J., Griffiths, B., Goodfield, M., Snaith, M. and Lindsey, N. J. (2003) Clinical & Experimental Immunology, Vol. 131, No. 1, pp. 182-189.en
dc.identifier.urihttp://hdl.handle.net/10454/4027
dc.descriptionnoen
dc.description.abstractCombinatorial antibody libraries were constructed from the spleen of a patient with concomitant systemic lupus erythematosus and idiopathic thrombocytopenia. Following selection of the libraries with DNA, a panel of 15 anti-DNA Fabs was isolated. Sequence analysis of these antibodies coupled with measurements of their affinities for ss- and dsDNA were used to investigate the role of somatic mutation in affinity maturation of the anti-DNA response. Examination of the germline genes used by these Fabs supports previous studies that suggest there is no restriction of the gene usage in the anti-DNA response. However, data are presented indicating that VH3 genes and the A27 V¿ paired with the J¿1 may be over-expressed in the anti-DNA repertoire. Analysis of the role of somatic mutation in increasing affinity for DNA indicates that affinity maturation has occurred and suggests that the CDR1 and CDR2 of the heavy chain are of importance in this process.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.isreferencedbyhttp://dx.doi.org/10.1046/j.1365-2249.2003.02026.xen
dc.subjectHuman spleenen
dc.subjectLupus erythematosusen
dc.subjectAutoantibodiesen
dc.subjectAutoimmunityen
dc.subjectSomatic mutationen
dc.titleThe role of somatic mutation in determining the affinity of anti-DNA antibodies.en
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionpublished version paperen


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