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dc.contributor.authorDavies, Gareth C.*
dc.contributor.authorThornton, M. Julie*
dc.contributor.authorJenner, Tracey J.*
dc.contributor.authorChen, Yi-Ju*
dc.contributor.authorHansen, J.B.*
dc.contributor.authorCarr, R.D.*
dc.contributor.authorRandall, Valerie A.*
dc.date.accessioned2009-11-30T14:38:46Z
dc.date.available2009-11-30T14:38:46Z
dc.date.issued2005
dc.identifier.citationDavies, G.C., Thornto, M.J., Jenner, T.J. and Randall, V.A. et al. (2005). Novel and established potassium channel openers stimulate hair growth in vitro: implications for their modes of action in hair follicles. Journal of Investigative Dermatology. Vol. 124, No. 4, pp. 686-694.en
dc.identifier.urihttp://hdl.handle.net/10454/4017
dc.descriptionNoen
dc.description.abstractAlthough ATP-sensitive potassium (K(ATP)) channel openers, e.g., minoxidil and diazoxide, can induce hair growth, their mechanisms require clarification. Improved drugs are needed clinically. but the absence of a good bioassay hampers research. K(ATP) channels from various tissues contain subtypes of the regulatory sulfonylurea receptor, SUR, and pore-forming, K(+) inward rectifier subunits, Kir6.X, giving differing sensitivities to regulators. Therefore, the in vitro effects of established potassium channel openers and inhibitors (tolbutamide and glibenclamide), plus a novel, selective Kir6.2/SUR1 opener, NNC 55-0118, were assessed on deer hair follicle growth in serum-free median without streptomycin. Minoxidil (0.1-100 microM, p<0.001), NNC 55-0118 (1 mM, p<0.01; 0.1, 10, 100 microM, p<0.001), and diazoxide (10 microM, p<0.01) increased growth. Tolbutamide (1 mM) inhibited growth (p<0.001) and abolished the effect of 10 microM minoxidil, diazoxide and NNC 55-0118; glibenclamide (10 microM) had no effect, but prevented stimulation by 10 microM minoxidil. Phenol red stimulated growth (p<0.001), but channel modulator responses remained unaltered. Thus, deer follicles offer a practical, ethically advantageous in vitro bioassay that reflects clinical responses in vivo. The results indicate direct actions of K(ATP) channel modulators within hair follicles via two types of channels, with SUR 1 and SUR 2, probably SUR2B, sulfonylurea receptors.en
dc.language.isoenen
dc.relation.isreferencedbyhttp://dx.doi.org/10.1111/j.0022-202X.2005.23643.xen
dc.subjectBioassayen
dc.subjectDiazoxideen
dc.subjectHair folliclesen
dc.subjectGlibenclamideen
dc.subjectMinoxidilen
dc.subjectNNC 55-0118en
dc.subjectOrgan cultureen
dc.subjectPhenol reden
dc.subjectTolbutamideen
dc.titleNovel and established potassium channel openers stimulate hair growth in vitromodes of action in hair follicles.: implications for theiren
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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