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dc.contributor.authorChoudry, Guzanfar A.*
dc.contributor.authorHamilton Stewart, P.A.*
dc.contributor.authorDouble, John A.*
dc.contributor.authorKrul, M.R.L.*
dc.contributor.authorNaylor, Brian*
dc.contributor.authorFlannigan, G. Michael*
dc.contributor.authorShah, Tariq K.*
dc.contributor.authorPhillips, Roger M.*
dc.date.accessioned2009-11-10T13:32:00Z
dc.date.available2009-11-10T13:32:00Z
dc.date.issued2001
dc.identifier.citationChoudry, G.A., Hamilton-Stewart, P., Double, J.A. and Phillips, R.M. et al. (2001). A novel strategy for NQO1 (NAD(P)H:quinone oxidoreductase, EC 1.6.99.2) mediated therapy of bladder cancer based on the pharmacological properties of EO9. British Journal of Cancer. Vol. 85, pp. 1137-1146.en
dc.identifier.urihttp://hdl.handle.net/10454/3889
dc.descriptionNoen
dc.description.abstractThe indolequinone EO9 demonstrated good preclinical activity but failed to show clinical efficacy against a range of tumours following intravenous drug administration. A significant factor in EO9's failure in the clinic has been attributed to its rapid pharmacokinetic elimination resulting in poor drug delivery to tumours. Intravesical administration of EO9 would circumvent the problem of drug delivery to tumours and the principal objective of this study is to determine whether or not bladder tumours have elevated levels of the enzyme NQO1 (NAD(P)H:quinone oxidoreductase) which plays a key role in activating EO9 under aerobic conditions. Elevated NQO1 levels in human bladder tumour tissue exist in a subset of patients as measured by both immunohistochemical and enzymatic assays. In a panel of human tumour cell lines, EO9 is selectively toxic towards NQO1 rich cell lines under aerobic conditions and potency can be enhanced by reducing extracellular pH. These studies suggest that a subset of bladder cancer patients exist whose tumours possess the appropriate biochemical machinery required to activate EO9. Administration of EO9 in an acidic vehicle could be employed to reduce possible systemic toxicity as any drug absorbed into the blood stream would become relatively inactive due to an increase in pH.en
dc.language.isoenen
dc.relation.isreferencedbyhttps://www.nature.com/articles/6692056en
dc.subjectBioreductive drugsen
dc.subjectEO9en
dc.subjectMitomycin Cen
dc.subjectBladder canceren
dc.subjectNQO1en
dc.titleA novel strategy for NQO1 (NAD(P)H:quinone oxidoreductase, EC 1.6.99.2) mediated therapy of bladder cancer based on the pharmacological properties of EO9.en
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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