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dc.contributor.authorWang, M.*
dc.contributor.authorObrenovitch, Tihomir P.*
dc.contributor.authorUrenjak, Jutta A.*
dc.date.accessioned2009-10-05T13:04:19Z
dc.date.available2009-10-05T13:04:19Z
dc.date.issued2003
dc.identifier.citationWang, M., Obrenovitch, T.P. and Urenjak, J.A. (2003). Effects of the nitric oxide donor, DEA/NO on cortical spreading depression. Neuropharmacology. Vol. 44, No. 7, pp. 949-957.en
dc.identifier.urihttp://hdl.handle.net/10454/3605
dc.descriptionNoen
dc.description.abstractCortical spreading depression (CSD) is a transient disruption of local ionic homeostasis that may promote migraine attacks and the progression of stroke lesions. We reported previously that the local inhibition of nitric oxide (NO) synthesis with N¿-nitro-L-arginine methyl ester (L-NAME) delayed markedly the initiation of the recovery of ionic homeostasis from CSD. Here we describe a novel method for selective, controlled generation of exogenous NO in a functioning brain region. It is based on microdialysis perfusion of the NO donor, 2-(N,N-diethylamino)-diazenolate-2-oxide (DEA/NO). As DEA/NO does not generate NO at alkaline pH, and as the brain has a strong acid-base buffering capacity, DEA/NO was perfused in a medium adjusted at alkaline (but unbuffered) pH. Without DEA/NO, such a microdialysis perfusion medium did not alter CSD. DEA/NO (1, 10 and 100 ¿M) had little effect on CSD by itself, but it reversed in a concentration-dependent manner the effects of NOS inhibition by 1 mM L-NAME. These data demonstrate that increased formation of endogenous NO associated with CSD is critical for subsequent, rapid recovery of cellular ionic homeostasis. In this case, the molecular targets for NO may be located either on brain cells to suppress mechanisms directly involved in CSD genesis, or on local blood vessels to couple flow to the increased energy demand associated with CSDen
dc.language.isoenen
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/S0028-3908(03)00082-0en
dc.subjectCortical spreading depressionen
dc.subjectIonic homeostasisen
dc.subjectNitric oxideen
dc.subjectNitric oxide synthaseen
dc.subjectN¿-nitro-L-arginine methyl ester (L-NAME)en
dc.subjectNO donor;en
dc.subjectMicrodialysisen
dc.subjectHigh extracellular potassiumen
dc.titleEffects of the nitric oxide donor, DEA/NO on cortical spreading depression.en
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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