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    High-speed microemulsion chromatography.

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    Publication date
    2001
    Author
    Mahuzier, P.E.
    Clark, Brian J.
    Bryant, S.M.
    Altria, K.D.
    Keyword
    Microemulsion electrokinetic chromatography
    High-speed separation
    Capillary electrophoresis
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    In previous reports of microemulsion electrokinetic chromatography (MEEKC), analysis times were typically in the order of 10 min as high-ionic strength buffers were used. These buffers produced high currents which limit the voltages which can be applied, therefore, analysis times could not be reduced. The primary cause of the high-ionic strength is the relatively high concentrations of surfactants required to form the microemulsion. The surfactant concentration can be lower when using an oil with a smaller surface tension. This preliminary study showed that migration times in MEEKC can be reduced to below 1 min by using a combination of an optimum microemulsion composition, high voltage, high temperature, short capillaries by injecting via the short end, or by simultaneously applying pressure and voltage. Long injection sequences and quantitation were found to be possible with minimum buffer depletion effects.
    URI
    http://hdl.handle.net/10454/3474
    Version
    No full-text available in the repository
    Citation
    Mahuzier, P.E., Clark, B.J., Bryant, S.M. and Altria, K.D. (2001). Electrophoresis. Vol. 22, No. 17, pp. 3819-3823.
    Link to publisher’s version
    http://dx.doi.org/10.1002/1522-2683(200109)22:17<3819::AID-ELPS3819>3.0.CO;2-E
    Type
    Article
    Collections
    Life Sciences Publications

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