cGMP-independent inhibition of integrin alphaIIbbeta3- mediated platelet adhesion and outside-in signalling by nitric oxide
| dc.contributor.author | Graham, Anne M | * |
| dc.contributor.author | Naseem, Khalid M. | * |
| dc.contributor.author | Oberprieler, Nikolaus G. | * |
| dc.contributor.author | Riba, Rocio | * |
| dc.contributor.author | Roberts, Wayne | * |
| dc.contributor.author | Homer-Vanniasinkam, Shervanthi | * |
| dc.date.accessioned | 2009-08-17T10:48:57Z | |
| dc.date.available | 2009-08-17T10:48:57Z | |
| dc.date.issued | 2007 | |
| dc.identifier.citation | Oberprieler, N.G., Roberts, W., Riba, R., Graham, A.M., Naseem, K.M. and Homer-Vanniasinkam, S. (2007). cGMP-independent inhibition of integrin alphaIIbbeta3- mediated platelet adhesion and outside-in signalling by nitric oxide. FEBS Letters. Vol. 581, No. 7, pp. 1529-34. | |
| dc.identifier.uri | http://hdl.handle.net/10454/3313 | |
| dc.description | No | |
| dc.description.abstract | We examined the influence of S-nitrosoglutathione (GSNO) on alpha(IIb)beta(3) integrin-mediated platelet adhesion to immobilised fibrinogen. GSNO induced a time- and concentration-dependent inhibition of platelet adhesion. Inhibition was cGMP-independent and associated with both reduced platelet spreading and protein tyrosine phosphorylation. To investigate the cGMP-independent effects of NO we evaluated integrin beta(3) phosphorylation. Adhesion to fibrinogen induced rapid phosphorylation of beta(3) on tyrosines 773 and 785, which was reduced by GSNO in a cGMP independent manner. Similar results were observed in suspended platelets indicating that NO-induced effects were independent of spreading-induced signalling. This is the first demonstration that NO directly regulates integrin beta(3) phosphorylation. | |
| dc.language.iso | en | |
| dc.subject | Nitric oxide | |
| dc.subject | cGMP | |
| dc.subject | Platelets | |
| dc.subject | Integrin ¿IIbß3 | |
| dc.subject | Tyrosine phosphorylation | |
| dc.title | cGMP-independent inhibition of integrin alphaIIbbeta3- mediated platelet adhesion and outside-in signalling by nitric oxide | |
| dc.status.refereed | Yes | |
| dc.type | Article | |
| dc.type.version | No full-text in the repository | |
| dc.identifier.doi | https://doi.org/10.1016/j.febslet.2007.02.072 | |
| dc.openaccess.status | closedAccess |