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    Biological Imaging with a Near-Field Optical Setup.

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    Publication date
    2003
    Author
    Denyer, Morgan C.T.
    Micheletto, R.
    Nakajima, K.
    Hara, M.
    Okazaki, S.
    Keyword
    NSOM
    SNOM
    Near field
    Optical microscope
    Fibers
    Biological sample
    Fluorescent
    Imaging
    Live samples
    Dynamic
    Peer-Reviewed
    Yes
    
    Metadata
    Show full item record
    Abstract
    Noncontact scanning near-field optical microscope (SNOM) systems can be used to optically resolve samples in atmospheric conditions at theoretical resolutions comparable to those of transmission electron microscope and atomic force microscope systems. SNOM systems are also increasingly used to image biological samples. In this study we custom built a SNOM system with the aim of further demonstrating the potential applications of near-field optical examination of biological material. In this study we were able to image both fixed whole-cell samples in air and liquid environments and live whole-cell samples in liquids. The images acquired were of a relatively low resolution, but this work has shown that SNOM systems can be used to monitor the dynamics of living cells at subnanometric resolutions in the z axis and for fluorescent imaging of whole cells in a liquid medium.
    URI
    http://hdl.handle.net/10454/3267
    Version
    No full-text available in the repository
    Citation
    Denyer, M.C.T., Micheletto, R., Nakajima, K. and Hara, M. et al (2003). Biological Imaging with a Near-Field Optical Setup. Journal of Nanoscience and Nanotechnology. Vol. 3, No. 6, pp. 496-502.
    Link to publisher’s version
    http://dx.doi.org/10.1166/jnn.2003.202
    Type
    Article
    Collections
    Life Sciences Publications

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