High Performance Liquid Chromatography Assay Method for Simultaneous Quantitation of Formoterol and Budesonide in Symbicort Turbuhaler
Publication date
2006Keyword
Simultaneous measurementHPLC chromatography
Corticosteroid
ß-Adrenergic receptor agonist
ß2-Adrenergic receptor
Agonist
Antiinflammatory agent
Bronchodilator
Antiasthma agent
Budesonide
Formoterol
Peer-Reviewed
YesOpen Access status
closedAccess
Metadata
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A sensitive and rapid high performance liquid chromatography method has been developed and used for the simultaneous determination of formoterol and budesonide in Symbicort Turbuhaler when assessing the aerodynamic characteristics of the emitted dose using Pharmacopoeial methods. This capability results in both time and cost saving. The mobile phase composition was acetonitrile-5 mM sodium dihydrogen orthophosphate, pH 3 (60: 40% v/v), and was passed at 1.5 ml min-1 through a C18 column with a UV detection (wavelength 214 nm). The method was shown to give good analytical performance in terms of linearity, precision (using phenylpropanolamine as an internal standard), sensitivity and solution stability. The intra-day precision for both formoterol and budesonide were 0.75% and 1.11%, respectively (n = 10). The limit of quantitation for formoterol was 10 ¿g L-1 and for budesonide was 120 ¿g L-1, and the limit of detection were 3 and 30 ¿g L-1, for both formoterol and budesonide, respectively. The method has been applied to determine the content of the emitted dose and the fine particle dose of Symbicort Turbuhaler.Version
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Assi, K.H., Tarsin, W. and Chrystyn, H. (2006). High Performance Liquid Chromatography Assay Method for Simultaneous Quantitation of Formoterol and Budesonide in Symbicort Turbuhaler. Journal of Pharmaceutical and Biomedical Analysis. Vol. 41, No. 1, pp. 325-328.Link to Version of Record
https://doi.org/10.1016/j.jpba.2005.11.015Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1016/j.jpba.2005.11.015