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    Production of monoclonal antibodies to sugarcane yellow leaf virus using recombinant read-through protein.

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    Publication date
    2009-07-21T13:13:32Z
    Author
    Coates, David
    Danks, C.
    Korimbocus, J.
    Preston, S.
    Boonham, N.
    Barker, I.
    Keyword
    Vertebrata
    Mammalia
    Rodentia
    Virus
    Diagnostic
    Technique ELISA
    Hybridome
    Virus recombinant
    Anticorps monoclonal
    Sugarcane yellowleaf
    Luteoviridae
    Show allShow less
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Yellow leaf syndrome (YLS) of sugarcane is associated with sugarcane yellow leaf virus (SCYLV), a member of the family Luteoviridae. A fragment of the coat protein and readthrough domain of SCYLV was expressed in a bacterial expression system. The resulting protein was purified and used to immunize mice for monoclonal antibody (MAb) production. Two MAbs, 3A2E3 and 2F7H5, were selected following the screening of hybridoma cells using both plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) and tissue blot immunoassay (TBIA). These MAbs can be incorporated into the TBIA assay currently used for the routine detection of SCYLV but could not be used in triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA). The two antibodies selected have slightly different specificities. Antibody 3A2E3 gave equivalent results to a polyclonal antiserum (raised to purified virus) in comparative testing using TBIA. The MAbs produced should provide a widely available, uniform reagent for SCYLV diagnosis with the potential to help manage YLS.
    URI
    http://hdl.handle.net/10454/3088
    Version
    published version paper
    Citation
    Coates, D, Danks, C., Korimbocus, J. and Preston S. et al. (2002). Production of monoclonal antibodies to sugarcane yellow leaf virus using recombinant read-through protein. Journal of Phytopathology. Vol. 150, No. 8-9, pp. 488-494.
    Link to publisher’s version
    http://www3.interscience.wiley.com/cgi-bin/fulltext/118921940/PDFSTART
    Type
    Article
    Collections
    Life Sciences Publications

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