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dc.contributor.authorWood, John M.
dc.contributor.authorGibbons, Nick C.
dc.contributor.authorAbou Elloof, M.M.
dc.contributor.authorSchallreuter, Karin U.
dc.date.accessioned2009-07-14T08:59:46Z
dc.date.available2009-07-14T08:59:46Z
dc.date.issued2009-07-14T08:59:46Z
dc.identifier.citationSchallreuter, K. U., Gibbons, N. C., Abou Elloof, M. M., Wood, J. M. (2007). Hydrogen peroxide-mediated oxidative stress disrupts calcium binding on calmodulin: more evidence for oxidative stress in vitiligo. Biochemical and Biophysical Research Communications. Vol. 360, No. 1.en
dc.identifier.urihttp://hdl.handle.net/10454/3001
dc.descriptionNoen
dc.description.abstractPatients with acute vitiligo have low epidermal catalase expression/activities and accumulate 10 -3 M H 2O 2. One consequence of this severe oxidative stress is an altered calcium homeostasis in epidermal keratinocytes and melanocytes. Here, we show decreased epidermal calmodulin expression in acute vitiligo. Since 10 -3M H 2O 2 oxidises methionine and tryptophan residues in proteins, we examined calcium binding to calmodulin in the presence and absence of H 2O 2 utilising 45calcium. The results showed that all four calcium atoms exchanged per molecule of calmodulin. Since oxidised calmodulin looses its ability to activate calcium ATPase, enzyme activities were followed in full skin biopsies from lesional skin of patients with acute vitiligo (n = 6) and healthy controls (n = 6). The results yielded a 4-fold decrease of ATPase activities in the patients. Computer simulation of native and oxidised calmodulin confirmed the loss of all four calcium ions from their specific EF-hand domains. Taken together H 2O 2-mediated oxidation affects calcium binding in calmodulin leading to perturbed calcium homeostasis and perturbed L-phenylalanine-uptake in the epidermis of acute vitiligo.en
dc.language.isoenen
dc.relation.isreferencedby10.1016/j.bbrc.2007.05.218en
dc.subjectAnimal Cellsen
dc.subjectBiological Stressen
dc.subjectBiopsyen
dc.subjectCalcium ionsen
dc.subjectCalmodulinen
dc.subjectCatalaseen
dc.subjectCompututerised simulationen
dc.subjectEnzyme activityen
dc.subjectGene Regulationen
dc.subjectHomeostatsisen
dc.subjectHydrogen peroxideen
dc.subjectMelaninen
dc.subjectMethionineen
dc.subjectOxidationen
dc.subjectPhenylalanineen
dc.subjectTryptophanen
dc.titleHydrogen peroxide-mediated oxidative stress disrupts calcium binding on calmodulin: more evidence for oxidative stress in vitiligoen
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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