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    Hydrogen peroxide-mediated oxidative stress disrupts calcium binding on calmodulin: more evidence for oxidative stress in vitiligo

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    Publication date
    2009-07-14T08:59:46Z
    Author
    Wood, John M.
    Gibbons, Nick C.
    Abou Elloof, M.M.
    Schallreuter, Karin U.
    Keyword
    Animal Cells
    Biological Stress
    Biopsy
    Calcium ions
    Calmodulin
    Catalase
    Compututerised simulation
    Enzyme activity
    Gene Regulation
    Homeostatsis
    Hydrogen peroxide
    Melanin
    Methionine
    Oxidation
    Phenylalanine
    Tryptophan
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    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Patients with acute vitiligo have low epidermal catalase expression/activities and accumulate 10 -3 M H 2O 2. One consequence of this severe oxidative stress is an altered calcium homeostasis in epidermal keratinocytes and melanocytes. Here, we show decreased epidermal calmodulin expression in acute vitiligo. Since 10 -3M H 2O 2 oxidises methionine and tryptophan residues in proteins, we examined calcium binding to calmodulin in the presence and absence of H 2O 2 utilising 45calcium. The results showed that all four calcium atoms exchanged per molecule of calmodulin. Since oxidised calmodulin looses its ability to activate calcium ATPase, enzyme activities were followed in full skin biopsies from lesional skin of patients with acute vitiligo (n = 6) and healthy controls (n = 6). The results yielded a 4-fold decrease of ATPase activities in the patients. Computer simulation of native and oxidised calmodulin confirmed the loss of all four calcium ions from their specific EF-hand domains. Taken together H 2O 2-mediated oxidation affects calcium binding in calmodulin leading to perturbed calcium homeostasis and perturbed L-phenylalanine-uptake in the epidermis of acute vitiligo.
    URI
    http://hdl.handle.net/10454/3001
    Version
    No full-text available in the repository
    Citation
    Schallreuter, K. U., Gibbons, N. C., Abou Elloof, M. M., Wood, J. M. (2007). Hydrogen peroxide-mediated oxidative stress disrupts calcium binding on calmodulin: more evidence for oxidative stress in vitiligo. Biochemical and Biophysical Research Communications. Vol. 360, No. 1.
    Link to publisher’s version
    10.1016/j.bbrc.2007.05.218
    Type
    Article
    Collections
    Life Sciences Publications

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