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    Autocrine catecholamine biosynthesis and the b- adrenoceptor signal is present in Human Epidermal Melanocytes

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    Publication date
    2009-07-13T15:59:30Z
    Author
    Schallreuter, Karin U.
    Gillbro, Johanna M.
    Hibberts, Nigel A.
    Marles, Lee K.
    Keyword
    B- 2-adrenoceptors
    cAMP
    Catecholamine
    Biosynthesis
    Melanocytes
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Earlier it has been shown that human proliferating/undifferentiated basal keratinocytes hold the full capacity for autocrine catecholamine synthesis/degradation and express b2-adrenoceptors (b2-AR). In this report, we show that human melanocytes also express all of the mRNA and enzymes for autocrine synthesis of norepinephrine but fail to produce epinephrine. So far, it was established that human melanocytes express b1-AR which are induced by norepinephrine yielding the inosine triphosphate diacylglycerol signal. The presence of catecholamine synthesis and the b2-AR signal escaped definition at that time. Using RT-PCR, immunofluorescence and radioligand binding with the b2-AR antagonist (-)-[3H]CGP 12177, we show here that human melanocytes express functional b2-AR (4230 receptors per cell) with a Bmax at 129.3 and a KD of 3.19 nM but lack b1-AR expression. 2-AR stimulation with epinephrine 10-6 M and salbutamol 10-6¿10-5 M yielded a strong cyclic adenosine monophospate (cAMP) response in association with upregulated melanin production. Taken together these results indicate that the biosynthesis and release of epinephrine (10-6 M) by surrounding keratinocytes can provide the cAMP response leading to melanogenesis in melanocytes via the b2-AR signal. Moreover, the discovery of this catecholaminergic cAMP response in melanocytes adds a new source for this important second messenger in melanogenesis.
    URI
    http://hdl.handle.net/10454/2995
    Version
    No full-text available in the repository
    Citation
    Gillbro, J. M., Marles, Lee K., Hibberts, N. A., Schallreuter, K. U. (2004). Autocrine catecholamine biosynthesis and the b- adrenoceptor signal is present in Human Epidermal Melanocytes. Journal of Investigative Dermatology. Vol. 123, pp. 346-353.
    Link to publisher’s version
    https://doi.org/10.1111/j.0022-202X.2004.23210.x
    Type
    Article
    Collections
    Life Sciences Publications

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