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dc.contributor.authorRandall, Valerie A.*
dc.contributor.authorHibberts, Nigel A.*
dc.contributor.authorStreet, T.*
dc.contributor.authorThornton, M. Julie*
dc.date.accessioned2009-07-08T07:32:33Z
dc.date.available2009-07-08T07:32:33Z
dc.date.issued2001
dc.identifier.citationRandall, V., Hibberts, N.A., Street, T. and Thornton, M.J. (2001) Androgen receptors are only present in mesenchyme-derived dermal papilla cells of red deer (Cervus elaphus) neck follicles when raised androgens induce a mane in the breeding season. Journal of Endocrinology. Vol. 168, No. 3, pp. 401-408.en
dc.identifier.urihttp://hdl.handle.net/10454/2946
dc.descriptionNoen
dc.description.abstractRed deer stags produce an androgen-dependent mane of long hairs only in the breeding season; in the non-breeding season, when circulating androgen levels are low, the neck hair resembles the rest of the coat. This study was designed to determine whether androgen receptors are present in deer follicles throughout the year or only in the mane (neck) follicles when circulating testosterone levels are high in the breeding season. Although androgens regulate much human hair growth the mechanisms are not well understood; they are believed to act on the hair follicle epithelium via the mesenchyme-derived dermal papilla. The location of androgen receptors in the follicle was investigated by immunohistochemistry and androgen binding was measured biochemically in cultured dermal papilla cells derived from mane and flank follicles during the breeding season and from neck follicles during the non-breeding season. Immunohistochemistry of frozen skin sections using a polyclonal antibody to the androgen receptor localised nuclear staining only in the dermal papilla cells of mane follicles. Saturation analysis assays of 14 primary dermal papilla cell lines using [(3)H]-mibolerone demonstrated high-affinity, low-capacity androgen receptors were present only in mane (breeding season neck) cells; competition studies with other steroids confirmed the specificity of the receptors. Androgen receptors were not detectable in cells from either the breeding season flank nor the non-breeding season neck follicles. The unusual biological model offered by red deer of androgen-dependent hair being produced on the neck in the breeding, but not the non-breeding season, has allowed confirmation that androgen receptors are required in follicle dermal papilla cells for an androgen response; this concurs with previous human studies. In addition, the absence of receptors in the non-breeding season follicles demonstrates that receptors are not expressed unless the follicle is responding to androgens. Androgen receptors may be induced in mane follicles by seasonal changes in circulating hormone(s).en
dc.language.isoenen
dc.relation.isreferencedbyhttp://joe.endocrinology-journals.org/cgi/reprint/168/3/401en
dc.subjectBreeding seasonen
dc.subjectHormonal receptoren
dc.subjectAndrogenen
dc.subjectHair follicleen
dc.subjectBinding siteen
dc.subjectCervus elaphusen
dc.subjectHairen
dc.subjectSex steroid hormoneen
dc.subjectArtiodactylaen
dc.subjectUngulataen
dc.subjectMammaliaen
dc.subjectVertebrataen
dc.titleAndrogen receptors are only present in mesenchyme-derived dermal papilla cells of red deer (Cervus elaphus) neck follicles when raised androgens induce a mane in the breeding seasonen
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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