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dc.contributor.authorYazici, Zihni A.*
dc.contributor.authorRaschi, E.*
dc.contributor.authorPatel, Anjana*
dc.contributor.authorTestoni, C.*
dc.contributor.authorBorghi, M.O.*
dc.contributor.authorGraham, Anne M*
dc.contributor.authorMeroni, P.L.*
dc.contributor.authorLindsey, Nigel J.*
dc.date.accessioned2009-07-06T10:38:35Z
dc.date.available2009-07-06T10:38:35Z
dc.date.issued2001
dc.identifier.citationYazici ZA, Raschi E, Patel A, Testoni C, Borghi MO, Graham AM, Meroni PL and Lindsey N (2001) Human monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro. International Immunology. 13(3): 349-357.
dc.identifier.urihttp://hdl.handle.net/10454/2937
dc.descriptionNo
dc.description.abstractOur objectives were to obtain monoclonal anti-endothelial cell antibodies (AECA) from systemic lupus erythematosus (SLE) patients, to characterize their antigen specificity, and their capability to induce a pro-inflammatory and pro-adhesive endothelial phenotype, and to investigate the mechanism of endothelial cell (EC) activation in vitro. Monoclonal IgG AECA were generated by hybridoma formation with human SLE B cells. Antigen specificity was characterized by immunoblotting with enriched cell membrane fractions, by cytofluorimetry and by cell solid-phase ELISA. Endothelial activation was evaluated by measuring increases in U937 cell adhesiveness, adhesion molecule (E-selectin and ICAM-1) expression and IL-6 production. In addition, mechanisms of endothelial activation were investigated by assessment of NF-B by measuring the loss of its inhibitor I-B. mAb E-3 bound live EC and recognized a 42 kDa EC membrane protein, it enhanced U937 adhesiveness, E-selectin and ICAM-1 expression and IL-6 production, and caused the loss of I-B. We conclude this is the first in vitro demonstration that a human monoclonal AECA from a SLE patient reacts with a constitutive endothelial membrane antigen and induces a pro-inflammatory endothelial phenotype through NF-B activation.
dc.language.isoen
dc.subjectAdhesion molecules
dc.subjectAnti-endothelial cell antibody
dc.subjectCytokines
dc.subjectSystemic lupus erythematosus
dc.titleHuman monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro.
dc.status.refereedYes
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1093/intimm/13.3.349
dc.openaccess.statusclosedAccess


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