Human monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro.
Publication date
2001Author
Yazici, Zihni A.Raschi, E.
Patel, Anjana
Testoni, C.
Borghi, M.O.
Graham, Anne M
Meroni, P.L.
Lindsey, Nigel J.
Peer-Reviewed
Yes
Metadata
Show full item recordAbstract
Our objectives were to obtain monoclonal anti-endothelial cell antibodies (AECA) from systemic lupus erythematosus (SLE) patients, to characterize their antigen specificity, and their capability to induce a pro-inflammatory and pro-adhesive endothelial phenotype, and to investigate the mechanism of endothelial cell (EC) activation in vitro. Monoclonal IgG AECA were generated by hybridoma formation with human SLE B cells. Antigen specificity was characterized by immunoblotting with enriched cell membrane fractions, by cytofluorimetry and by cell solid-phase ELISA. Endothelial activation was evaluated by measuring increases in U937 cell adhesiveness, adhesion molecule (E-selectin and ICAM-1) expression and IL-6 production. In addition, mechanisms of endothelial activation were investigated by assessment of NF-B by measuring the loss of its inhibitor I-B. mAb E-3 bound live EC and recognized a 42 kDa EC membrane protein, it enhanced U937 adhesiveness, E-selectin and ICAM-1 expression and IL-6 production, and caused the loss of I-B. We conclude this is the first in vitro demonstration that a human monoclonal AECA from a SLE patient reacts with a constitutive endothelial membrane antigen and induces a pro-inflammatory endothelial phenotype through NF-B activation.Version
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Yazici ZA, Raschi E, Patel A, Testoni C, Borghi MO, Graham AM, Meroni PL and Lindsey N (2001) Human monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro. International Immunology. 13(3): 349-357.Link to Version of Record
https://doi.org/10.1093/intimm/13.3.349Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1093/intimm/13.3.349