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    Human monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro.

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    Publication date
    2001
    Author
    Yazici, Zihni A.
    Raschi, E.
    Patel, Anjana
    Testoni, C.
    Borghi, M.O.
    Graham, Anne M.
    Meroni, P.L.
    Lindsey, Nigel J.
    Keyword
    Adhesion molecules
    Anti-endothelial cell antibody
    Cytokines
    Systemic lupus erythematosus
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Our objectives were to obtain monoclonal anti-endothelial cell antibodies (AECA) from systemic lupus erythematosus (SLE) patients, to characterize their antigen specificity, and their capability to induce a pro-inflammatory and pro-adhesive endothelial phenotype, and to investigate the mechanism of endothelial cell (EC) activation in vitro. Monoclonal IgG AECA were generated by hybridoma formation with human SLE B cells. Antigen specificity was characterized by immunoblotting with enriched cell membrane fractions, by cytofluorimetry and by cell solid-phase ELISA. Endothelial activation was evaluated by measuring increases in U937 cell adhesiveness, adhesion molecule (E-selectin and ICAM-1) expression and IL-6 production. In addition, mechanisms of endothelial activation were investigated by assessment of NF-B by measuring the loss of its inhibitor I-B. mAb E-3 bound live EC and recognized a 42 kDa EC membrane protein, it enhanced U937 adhesiveness, E-selectin and ICAM-1 expression and IL-6 production, and caused the loss of I-B. We conclude this is the first in vitro demonstration that a human monoclonal AECA from a SLE patient reacts with a constitutive endothelial membrane antigen and induces a pro-inflammatory endothelial phenotype through NF-B activation.
    URI
    http://hdl.handle.net/10454/2937
    Version
    No full-text available in the repository
    Citation
    Yazici ZA, Raschi E, Patel A, Testoni C, Borghi MO, Graham AM, Meroni PL and Lindsey N (2001) Human monoclonal anti-endothelial cell IgG-derived from a systemic lupus erythematosus patient binds and activates human endotheliium in vitro. International Immunology. 13(3): 349-357.
    Link to publisher’s version
    https://doi.org/10.1093/intimm/13.3.349
    Type
    Article
    Collections
    Life Sciences Publications

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