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dc.contributor.authorJackson, K.A.*
dc.contributor.authorEdwards-Jones, V.*
dc.contributor.authorSutton, Chris W.*
dc.contributor.authorFox, A.J.*
dc.date.accessioned2009-06-09T10:28:33Z
dc.date.available2009-06-09T10:28:33Z
dc.date.issued2005
dc.identifier.citationJackson, K.A., Edwards-Jones, V., Sutton, C.W. and Fox, A.J. (2005). Optimisation of intact cell MALDI method for fingerprinting of methicillin-resistant Staphylococcus aureus. Journal of Microbiological Methods. Vol. 62, No. 3, pp. 273-284.en
dc.identifier.urihttp://hdl.handle.net/10454/2788
dc.descriptionNoen
dc.description.abstractThe use of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry on intact cell microorganisms, Intact Cell MALDI (ICM), has been shown by numerous workers to yield effective species level identification. Early work highlighted the significant effect that variation in culture media, incubation conditions and length of incubation had on the spectra produced. Therefore, in order to achieve reliable and reproducible species level identification and sub-typing of microorganisms from ICM fingerprints, it has been essential to develop standardised methods. For methicillin-resistant Staphylococcus aureus (MRSA), a major nosocomial pathogen, we have developed such a standardised method. In this paper we present the experimental parameters, namely, the incubation period, the number of passages required from lyophilised or stored isolates, the method of deposition of the bacterial cells, the concentration of matrix solution, the drying time of bacterial cells prior to the addition of the matrix solution, the time between preparation of the bacterial/matrix sample and analysis and the MALDI pulsed extraction setting, which were considered during the development of defined methods.en
dc.language.isoenen
dc.relation.isreferencedbyhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T30-4GJM3F2-1&_user=122861&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000010080&_version=1&_urlVersion=0&_userid=122861&md5=837a11f3107a5adc7510d62d2092d72ben
dc.subjectMatrix assisted laser desorption ionizationen
dc.subjectStaphylococcus aureusen
dc.subjectBacteriaen
dc.subjectMicrococcalesen
dc.subjectMicrococcaceaeen
dc.subjectAntibioticen
dc.subjectAntibacterial agenten
dc.subjectFingerprint methoden
dc.subjectResistanceen
dc.subjectMeticillinen
dc.titleOptimisation of intact cell MALDI method for fingerprinting of methicillin-resistant Staphylococcus aureus.en
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text available in the repositoryen


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