Show simple item record

dc.contributor.authorForbes, Robert T.*
dc.contributor.authorApperley, D.C.*
dc.contributor.authorSuihko, E.J.*
dc.date.accessioned2009-05-18T10:47:49Z
dc.date.available2009-05-18T10:47:49Z
dc.date.issued2005
dc.identifier.citationForbes, R.T., Apperley, D.C. and Suihko, E.J. (2005). A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles. European Journal of Pharmaceutical Sciences. Vol. 25, No. 1. pp. 105-112.en
dc.identifier.urihttp://hdl.handle.net/10454/2650
dc.descriptionNoen
dc.description.abstractMolecular mobility and physical form of co-spray-dried sugar:lysozyme formulations were evaluated. Co-spray-dried trehalose:lysozyme and sucrose:lysozyme formulations in 1:9, 1:1 and 9:1 ratios (w:w) were stored at 0% RH and 75% RH for 5-6 days. Molecular mobility and physical form of the co-spray-dried formulations after storage were determined by using 13C and 1H solid-state NMR as well as X-ray powder diffractometry. The results showed that increasing sugar content in co-spray-dried formulations stored at 0% RH decreased molecular mobility of the amorphous formulations indicating a close association of the protein and sugar. Exposure of sugar:lysozyme 1:1 and 9:1 formulations to 75% RH led to separation of sugar and protein phases, where the sugar phase was crystalline. The intimate sugar:lysozyme interaction of the formulations stored at 0% RH and the phase separation of the sugar-rich formulations stored at 75% RH were also confirmed by using 13C solid-state NMR spin-lattice relaxation time-filter (T1-filter) measurements. The propensity of sucrose and trehalose to crystallise was similar; however, the results suggest that part of the sugar in the phase-separated formulations remained amorphous and in close association with lysozyme.en
dc.language.isoenen
dc.subjectProtein formulationen
dc.subjectSpray-dryingen
dc.subjectSolid-state NMRen
dc.subjectMolecular mobilityen
dc.subjectPhase separationen
dc.titleA solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particlesen
dc.status.refereedYesen
dc.typeArticleen
dc.type.versionNo full-text in the repositoryen
dc.identifier.doihttps://doi.org/10.1016/j.ejps.2005.02.002


This item appears in the following Collection(s)

Show simple item record