BRADFORD SCHOLARS

    • Sign in
    View Item 
    •   Bradford Scholars
    • Life Sciences
    • Life Sciences Publications
    • View Item
    •   Bradford Scholars
    • Life Sciences
    • Life Sciences Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Bradford ScholarsCommunitiesAuthorsTitlesSubjectsPublication DateThis CollectionAuthorsTitlesSubjectsPublication Date

    My Account

    Sign in

    HELP

    Bradford Scholars FAQsCopyright Fact SheetPolicies Fact SheetDeposit Terms and ConditionsDigital Preservation Policy

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Publication date
    2005
    Author
    Forbes, Robert T.
    Apperley, D.C.
    Suihko, E.J.
    Keyword
    Protein formulation
    Spray-drying
    Solid-state NMR
    Molecular mobility
    Phase separation
    Peer-Reviewed
    Yes
    
    Metadata
    Show full item record
    Abstract
    Molecular mobility and physical form of co-spray-dried sugar¿lysozyme formulations were evaluated. Co-spray-dried trehalose:lysozyme and sucrose:lysozyme formulations in 1:9, 1:1 and 9:1 ratios (w:w) were stored at 0% RH and 75% RH for 5¿6 days. Molecular mobility and physical form of the co-spray-dried formulations after storage were determined by using 13C and 1H solid-state NMR as well as X-ray powder diffractometry. The results showed that increasing sugar content in co-spray-dried formulations stored at 0% RH decreased molecular mobility of the amorphous formulations indicating a close association of the protein and sugar. Exposure of sugar¿lysozyme 1:1 and 9:1 formulations to 75% RH led to separation of sugar and protein phases, where the sugar phase was crystalline. The intimate sugar¿lysozyme interaction of the formulations stored at 0% RH and the phase separation of the sugar-rich formulations stored at 75% RH were also confirmed by using 13C solid-state NMR spin-lattice relaxation time-filter (T1-filter) measurements. The propensity of sucrose and trehalose to crystallise was similar; however, the results suggest that part of the sugar in the phase-separated formulations remained amorphous and in close association with lysozyme.
    URI
    http://hdl.handle.net/10454/2650
    Version
    No full-text available in the repository
    Citation
    Forbes, R.T., Apperley, D.C. and Suihko, E.J. (2005). A solid-state NMR study of molecular mobility and phase separation in co-spray-dried protein-sugar particles. European Journal of Pharmaceutical Sciences. Vol. 25, No. 1. pp. 105-112.
    Link to publisher’s version
    http://www.sciencedirect.com/science?_ob=PdfDownloadURL&_uoikey=B6T25-4FK42HJ-3&_tockey=%23toc%234909%232005%23999749998%23593773%23FLA%23&_orig=search&_acct=C000010080&_version=1&_userid=122861&md5=a525bd4d889a1ff5c9aa418004a55f82
    Type
    Article
    Collections
    Life Sciences Publications

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.