Show simple item record

dc.contributor.advisorBotchkarev, Vladimir A.
dc.contributor.advisorMardaryev, Andrei N.
dc.contributor.advisorFessing, Michael Y.
dc.contributor.advisorPoterlowicz, Krzysztof
dc.contributor.authorFatima, Iqra
dc.date.accessioned2024-04-04T14:31:59Z
dc.date.available2024-04-04T14:31:59Z
dc.date.issued2022
dc.identifier.urihttp://hdl.handle.net/10454/19871
dc.description.abstractBeing the longest-lived rodent, naked mole-rats (NMR; Heterocephalus glaber) are an exceptional model for biogerontological research. However, unlike other rodents, not much is known about their wound healing process. To investigate that, full-thickness wounds were created in the back skin of naked mole rats. Our initial data confirmed that wound closure in NMR skin was achieved primarily by reepithelialization and granulation tissue formation, with only ~26% wound contraction, making them an excellent model to study human cutaneous wound healing. Similar to mice and human skin, changes in wound epithelial tongue included progressive enlargement of wound epithelium, increased proliferation and changes in the expression pattern of epidermal markers including K14, K17, integrin α6 and E-cadherin. Further analysis revealed characteristics of reduced scarring in NMR wounds including low collagen I to III ratio, increased HA expression (HMW) and increased fibronectin expression. Transcriptional profiling of TGFβ isoforms and different pro/anti-inflammatory cytokines revealed a balance in the expression and repression of different cytokines, potentially contributing into reduced scarring. Comparison of RNA-seq data from NMR and human fullthickness wounds revealed a delay in the activation of important biological processes and pathways in NMR skin in response to injury. Further analysis based on cultured human and NMR cells revealed differential regulation of TGFβ signalling pathway between both species. 3-D collagen gel contraction assay revealed that NMR fibroblast showed noticeable contraction but independently of TGFβ treatment, while human fibroblast showed marked increased in gel contraction in the presence of TGFβ. In conclusion, NMR can serve as a very useful model to study human cutaneous wound healing. The reduced scarring in NMR could be a result of multiple factors including HMW-HA, balanced cytokine expression and differential regulation of different TGFβ cytokines as observed in the in vitro studies.en
dc.language.isoenen_US
dc.rights<a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>.eng
dc.subjectNaked mole ratsen_US
dc.subjectSkinen_US
dc.subjectWound healingen_US
dc.subjectScaren_US
dc.subjectTGFβen_US
dc.subjectFibroblastsen_US
dc.subjectRe-epithelializationen_US
dc.subjectExtracellular matrixen_US
dc.subjectCutaneousen_US
dc.titleCharacterisation of Cutaneous Wound Healing Process in Naked Mole Ratsen_US
dc.type.qualificationleveldoctoralen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentSchool of Chemistry and Biosciences. Faculty of Life Sciencesen_US
dc.typeThesiseng
dc.type.qualificationnamePhDen_US
dc.date.awarded2022
refterms.dateFOA2024-04-04T14:31:59Z


Item file(s)

Thumbnail
Name:
15020149 Iqra Fatima Final Thesis ...
Size:
16.80Mb
Format:
PDF
Description:
PhD Thesis

This item appears in the following Collection(s)

Show simple item record