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dc.contributor.authorPresa, Daniela
dc.contributor.authorKhurram, S.A.
dc.contributor.authorZubir, A.Z.A.
dc.contributor.authorSwaroop, Sneha
dc.contributor.authorCooper, Patricia A.
dc.contributor.authorMorais, Goreti R.
dc.contributor.authorSadiq, Maria
dc.contributor.authorSutherland, Mark
dc.contributor.authorLoadman, Paul
dc.contributor.authorMcCaul, Jim
dc.contributor.authorShnyder, Steven
dc.contributor.authorPatterson, Laurence H.
dc.contributor.authorPors, Klaus
dc.date.accessioned2023-12-11T17:40:48Z
dc.date.accessioned2023-12-18T15:41:22Z
dc.date.available2023-12-11T17:40:48Z
dc.date.available2023-12-18T15:41:22Z
dc.date.issued2021-09
dc.identifier.citationPresa D, Khurram SA, Zubir AZA et al (2021) Cytochrome P450 isoforms 1A1, 1B1 AND 2W1 as targets for therapeutic intervention in head and neck cancer. Scientific Reports. 11: 18930.en_US
dc.identifier.urihttp://hdl.handle.net/10454/19728
dc.descriptionYesen_US
dc.description.abstractEpidemiological studies have shown that head and neck cancer (HNC) is a complex multistage process that in part involves exposure to a combination of carcinogens and the capacity of certain drug-metabolising enzymes including cytochrome P450 (CYP) to detoxify or activate such carcinogens. In this study, CYP1A1, CYP1B1 and CYP2W1 expression in HNC was correlated with potential as target for duocarmycin prodrug activation and selective therapy. In the HNC cell lines, elevated expression was shown at the gene level for CYP1A1 and CYP1B1 whereas CYP2W1 was hardly detected. However, CYP2W1 was expressed in FaDu and Detroit-562 xenografts and in a cohort of human HNC samples. Functional activity was measured in Fadu and Detroit-562 cells using P450-Glo™ assay. Antiproliferative results of duocarmycin prodrugs ICT2700 and ICT2706 revealed FaDu and Detroit-562 as the most sensitive HNC cell lines. Administration of ICT2700 in vivo using a single dose of ICT2700 (150 mg/kg) showed preferential inhibition of small tumour growth (mean size of 60 mm3) in mice bearing FaDu xenografts. Significantly, our findings suggest a potential targeted therapeutic approach to manage HNCs by exploiting intratumoural CYP expression for metabolic activation of duocarmycin-based prodrugs such as ICT2700.en_US
dc.description.sponsorshipThe authors would like to thank Bradford Institute for Health Research for funding a PhD studentship to DP through a competitive scheme and Yorkshire Cancer Research programme Grant (B381PA) for supporting our cytochrome P450-focused drug discovery research.en_US
dc.language.isoenen_US
dc.rights© The Author(s) 2021. Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectHead and neck cancer (HNC)en_US
dc.subjectCytochrome P450 (CYP)en_US
dc.subjectTherapeutic interventionen_US
dc.titleCytochrome P450 isoforms 1A1, 1B1 AND 2W1 as targets for therapeutic intervention in head and neck canceren_US
dc.status.refereedYesen_US
dc.date.Accepted2021-08-25
dc.date.application2021-09-23
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-021-98217-z
dc.rights.licenseCC-BYen_US
dc.date.updated2023-12-11T17:40:51Z
refterms.dateFOA2023-12-18T15:42:02Z
dc.openaccess.statusopenAccessen_US


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