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Publication date
17/10/2023Author
Soni, P.Edwards, H.
Anupom, T.
Rahman, M.
Lesanpezeshki, L.
Blawzdziewicz, J.
Cope, H.
Gharahdaghi, N.
Scott, D.
Toh, L.S.
Williams, P.M.
Etheridge, T.
Szewczyk, N.
Willis, Craig R.G.
Vanapalli, S.A.
Keyword
C. elegansInternational Space Station
Astropharmacy
Dystrophin
Gene expression
Microgravity
Muscle atrophy
Muscle strength
Omics
Spaceflight
Rights
(c) 2023 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (https://creativecommons.org/licenses/by/4.0/)Peer-Reviewed
YesOpen Access status
openAccessAccepted for publication
2023-10-15
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Show full item recordAbstract
Background: Understanding and countering the well-established negative health consequences of spaceflight remains a primary challenge preventing safe deep space exploration. Targeted/personalized therapeutics are at the forefront of space medicine strategies, and cross-species molecular signatures now define the 'typical' spaceflight response. However, a lack of direct genotype-phenotype associations currently limits the robustness and, therefore, the therapeutic utility of putative mechanisms underpinning pathological changes in flight. Methods: We employed the worm Caenorhabditis elegans as a validated model of space biology, combined with 'NemaFlex-S' microfluidic devices for assessing animal strength production as one of the most reproducible physiological responses to spaceflight. Wild-type and dys-1 (BZ33) strains (a Duchenne muscular dystrophy (DMD) model for comparing predisposed muscle weak animals) were cultured on the International Space Station in chemically defined media before loading second-generation gravid adults into NemaFlex-S devices to assess individual animal strength. These same cultures were then frozen on orbit before returning to Earth for next-generation sequencing transcriptomic analysis. Results: Neuromuscular strength was lower in flight versus ground controls (16.6% decline, pVersion
Published versionCitation
Soni P, Edwards H, Anupom T et al (2023) Spaceflight Induces Strength Decline in Caenorhabditis elegans. Cells. 12(20): 2470.Link to Version of Record
https://doi.org/10.3390/cells12202470Type
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.3390/cells12202470