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dc.contributor.authorSun, K.
dc.contributor.authorWhiteside, Benjamin R.
dc.contributor.authorHebda, Michael J.
dc.contributor.authorFan, Y.
dc.contributor.authorZhang, Y.
dc.contributor.authorXie, Y.
dc.contributor.authorLiang, K.
dc.date.accessioned2023-09-25T11:39:32Z
dc.date.accessioned2023-10-06T14:29:40Z
dc.date.available2023-09-25T11:39:32Z
dc.date.available2023-10-06T14:29:40Z
dc.date.issued2023-06
dc.identifier.citationSun K, Whiteside B, Hebda M et al (2023) A low-cost and hand-hold PCR microdevice based on water-cooling technology. Biomedical Microdevices. 25: 12.en_US
dc.identifier.urihttp://hdl.handle.net/10454/19608
dc.descriptionYesen_US
dc.description.abstractPolymerase chain reaction (PCR) has become a powerful tool for detecting various diseases due to its high sensitivity and specificity. However, the long thermocycling time and the bulky system have limited the application of PCR devices in Point-of-care testing. Herein, we have proposed an efficient, low-cost, and hand-hold PCR microdevice, mainly including a control module based on water-cooling technology and an amplification module fabricated by 3D printing. The whole device is tiny and can be easily hand-held with a size of about 110 mm × 100 mm × 40 mm and a weight of about 300 g at a low cost of about $170.83. Based on the water-cooling technology, the device can efficiently perform 30 thermal cycles within 46 min at a heating/cooling rate of 4.0/8.1 ℃/s. To test our instrument, plasmid DNA dilutions were amplified with this device; the results demonstrate successful nucleic acid amplification of the …en_US
dc.language.isoenen_US
dc.publisherSpringer US
dc.rights© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023.en_US
dc.subjectPCR microdeviceen_US
dc.subjectNucleic acid amplificationen_US
dc.subjectWater-coolingen_US
dc.subjectPoint-of-care testingen_US
dc.titleA low-cost and hand-hold PCR microdevice based on water-cooling technologyen_US
dc.status.refereedYesen_US
dc.date.application2023-03-18
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.identifier.doihttps://doi.org/10.1007/s10544-023-00652-4
dc.rights.licenseUnspecifieden_US
dc.date.updated2023-09-25T11:39:33Z
refterms.dateFOA2023-10-06T14:30:29Z
dc.openaccess.statusopenAccessen_US
dc.date.accepted2023-03-08


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