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dc.contributor.authorTrevor, L.V.
dc.contributor.authorRiches-Suman, Kirsten
dc.contributor.authorMahajan, A.L.
dc.contributor.authorThornton, M. Julie
dc.date.accessioned2023-03-13T12:28:23Z
dc.date.accessioned2023-03-28T10:40:26Z
dc.date.available2023-03-13T12:28:23Z
dc.date.available2023-03-28T10:40:26Z
dc.date.issued2023-03
dc.identifier.citationTrevor LV, Riches-Suman K, Mahajan AL et al (2023) Stromal vascular fraction cells from individuals who have previously undergone radiotherapy retain their pro-wound healing properties. Journal of Clinical Medicine. 12(5): 2052.
dc.identifier.urihttp://hdl.handle.net/10454/19380
dc.descriptionYes
dc.description.abstractBeneficial effects have been observed following the transplant of lipoaspirates containing adipose-derived stem cells into chronic wounds caused by oncologic radiotherapy. It is not yet certain whether adipose-derived stem cells are resistant to radiation exposure. Therefore, the aims of this study were to isolate stromal vascular fraction from human breast tissue exposed to radiotherapy and determine the presence of adipose-derived stem cells. Stromal vascular fraction from irradiated donor tissue was compared to commercially sourced pre-adipocytes. Immunocytochemistry was used to determine the presence of adipose-derived stem cell markers. Conditioned media from stromal vascular fraction isolated from irradiated donors was used as a treatment in a scratch wound assay of dermal fibroblasts also isolated from irradiated donors and compared to pre-adipocyte conditioned media and serum free control. This is the first report of human stromal vascular fraction being cultured from previously irradiated breast tissue. Stromal vascular fraction conditioned media from irradiated donors had a similar effect in increasing the migration of dermal fibroblasts from irradiated skin to pre-adipocyte conditioned media from healthy donors. Therefore, the ability of adipose-derived stem cells in the stromal vascular fraction to stimulate dermal fibroblasts in wound healing appears to be preserved following radiotherapy. This study demonstrates that stromal vascular fraction from irradiated patients is viable, functional and may have potential for regenerative medicine techniques following radiotherapy.
dc.description.sponsorshipThis research was funded by a Bradford City FC Supporters Fellowship for L.V.T. administered through the Plastic Surgery and Burns Research Unit, University of Bradford.
dc.language.isoen
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
dc.subjectRadiotherapy
dc.subjectAdipose-derived stem cells
dc.subjectStromal vascular fraction
dc.subjectDermal fibroblasts
dc.subjectWound healing
dc.subjectReconstructive medicine
dc.subjectResearch Development Fund Publication Prize Award
dc.titleStromal vascular fraction cells from individuals who have previously undergone radiotherapy retain their pro-wound healing properties
dc.status.refereedYes
dc.date.Accepted03/03/2023
dc.date.application04/03/2023
dc.typeArticle
dc.type.versionPublished version
dc.description.publicnotesResearch Development Fund Publication Prize Award winner, Mar 2023.
dc.identifier.doihttps://doi.org/10.3390/jcm12052052
dc.rights.licenseCC-BY
dc.date.updated2023-03-13T12:28:25Z
refterms.dateFOA2023-03-28T10:40:58Z
dc.openaccess.statusopenAccess


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