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dc.contributor.advisorSefat, Farshid
dc.contributor.advisorYouseffi, Mansour
dc.contributor.authorBazgir, Morteza
dc.date.accessioned2022-11-01T16:42:12Z
dc.date.available2022-11-01T16:42:12Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10454/19195
dc.description.abstractAnnually, about 80,000 people die in the United Kingdom due to myocardial infarction, congestive heart failure, stroke, or from other diseases related to blood vessels. The current gold standard treatment for replacing the damaged blood vessel is by autograft procedure, during which the internal mammary artery (IMA) graft or saphenous vein graft (SVG) are usually employed. However, some limitations are associated with this type of treatment, such as lack of donor site and post-surgery problems that could negatively affect the patient’s health. Therefore, this present work aims to fabricate a synthetic blood vessel that mimics the natural arteries and to be used as an alternative method for blood vessel replacement. Polymeric materials intended to be used for this purpose must possess several characteristics including: (1) Polymers must be biocompatible; (2) Biodegradable with adequate degradation rate; (3) Must maintain its structural integrity throughout intended use; (4) Must have ideal mechanical properties; and (5) Must encourage and enhance the proliferation of the cells. The feasibility of using synthetic biodegradable polymers such as poly (ε- caprolactone) (PCL) and poly (lactide-co-glycolic acid) (PLGA) for fabricating tubular vascular grafts was extensively investigated in this work. Many fundamental experiments were performed to develop porous tissue- engineered polymeric membranes for vascular graft purposes through electrospinning technique to achieve the main aim. Electrospinning was selected since the scaffolds produced by this method usually resemble structural morphology similar to the extracellular matrix (ECM). Hence, four 6mm in diameter tubular shape vascular grafts PCL only, PLGA only, coaxial (core-PCL and shell-PLGA), and bilayer (inner layer-PCL and outer layer-PLGA) was designed and fabricated successfully. The structure and properties of each scaffold membrane were observed by scanning electron microscopy (SEM), and these scaffolds were fully characterized by Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), water contact angle measurements, mechanical tensile test, as well as cell culture studies were carried out by seeding human umbilical vein cells (HUVEC) and human vascular Fibroblast cells (HVF). Moreover, all polymeric grafts underwent degradation process, and the change in their morphological structure properties was studied over 12 weeks at room temperature. All scaffolds were also exposed to a controlled temperature of 37°C for four weeks, in phosphate-buffered saline solution (pH, 7.3). It was found that all scaffolds displayed exceptional fibre structure and excellent degradability with adequate steady weight-loss confirming the suitability of the fabricated scaffolds for tissue engineering applications. The coaxial and bilayer scaffolds degraded at a much slower (and steadier) rate than the singular PCL and PLGA tubular scaffolds. Coaxial grafts fabricated via coaxial needle showed an increase in their fibre diameter and pore size volume than other membranes, but also showed to have significant tensile strength, elongation at fracture, and Young’s modulus. To conclude, all scaffolds have demonstrated to be reliable to adhere and proliferate HUVEC, and HVF cells, but these cells were attracted to the PLGA membrane more than other fabricated membranes.en_US
dc.language.isoenen_US
dc.rights<a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>.eng
dc.subjectTissue engineering of vascular graft (TEVG)en_US
dc.subjectTubular vascular graft (TVG)en_US
dc.subjectPolycaprolactone (PCL)en_US
dc.subjectPoly (lactide-co-glycolic acid) (PLGA)en_US
dc.subjectDegradationen_US
dc.subjectElectrospinningen_US
dc.subjectNanofibresen_US
dc.subjectHuman umbilical vein endothelial cells (HUVEC)en_US
dc.subjectHuman vascular fibroblast cells (HVF)en_US
dc.subjectTensile testen_US
dc.subjectFabricationen_US
dc.subjectBiodegradable scaffoldsen_US
dc.subjectVascular tissue engineeringen_US
dc.titleFabrication, Characterisation and Optimisation of Biodegradable Scaffolds for Vascular Tissue Engineering Application of PCL and PLGA Electrospun Polymers for Vascular Tissue Engineeringen_US
dc.type.qualificationleveldoctoralen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentFaculty of Engineering & Informatics, Department of Biomedical and Electronics Engineeringen_US
dc.typeThesiseng
dc.type.qualificationnamePhDen_US
dc.date.awarded2021
refterms.dateFOA2022-11-01T16:42:12Z


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