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    Generation and Use of Functional Hydrogels That Can Rapidly Sample Infected Surfaces

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    Publication date
    2022-04
    Author
    Swift, Thomas
    Pinnock, A.
    Shivshetty, N.
    Pownall, David
    MacNeil, S.
    Douglas, I.
    Garg, P.
    Rimmer, Stephen
    Keyword
    Contact lens
    Pathogen diagnosis
    Medical device
    Specificity
    Amphotericin elisa
    Polymyxin elisa
    Highly branched polymers
    Microbe detection
    Functional hydrogels
    Cornea - infections
    Rights
    © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    Open Access status
    openAccess
    
    Metadata
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    Abstract
    This paper outlined our method for developing polymer-linked contact lens type materials for rapid detection and differentiation of Gram-positive, Gram-negative bacteria and fungi in infected corneas. It can be applied to both model synthetic or ex-vivo corneal models and has been successfully trialed in an initial efficacy tested animal study. First a hydrogel substrate for the swab material is selected, we have demonstrated selective swabs using a glycerol monomethacrylate hydrogel. Alternatively any commercial material with carboxylic acid functional groups is suitable but risks nonspecific adhesion. This is then functionalised via use of N-hydroxysuccinimide reaction with amine groups on the specified highly branched polymer ligand (either individually gram negative, gram positive or fungal binding polymers or a combination of all three can be employed for desired sensing application). The hydrogel is then cut into swabs suitable for sampling, used, and then the presence of gram positive, game negative and fungi are disclosed by the sequential addition of dyes (fluorescent vancomycin, fluorescein isothiocyanate and calcofluor white). In summary this method presents: Method to produce glycerol monomethacrylate hydrogels to minimize nonspecific binding Methods of attaching pathogen binding highly branched polymers to produce selective hydrogel swabs Method for disclosing bound pathogens to this swab using sequential dye addition
    URI
    http://hdl.handle.net/10454/19122
    Version
    Published version
    Citation
    Swift T, Pinnock A, Shivshetty N, et al (2022) Generation and Use of Functional Hydrogels That Can Rapidly Sample Infected Surfaces. MethodsX. 9: 101684
    Link to publisher’s version
    https://doi.org/10.1016/j.mex.2022.101684
    Type
    Article
    Collections
    Life Sciences Publications

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