Show simple item record

dc.contributor.authorCurtis, Mandi J.
dc.contributor.authorBeaumont, Julia
dc.contributor.authorElamin, F.
dc.contributor.authorWilson, Andrew S.
dc.contributor.authorKoon, Hannah E.C.
dc.date.accessioned2022-04-12T13:43:51Z
dc.date.accessioned2022-04-13T07:36:51Z
dc.date.available2022-04-12T13:43:51Z
dc.date.available2022-04-13T07:36:51Z
dc.date.issued2022-07-15
dc.identifier.citationCurtis MJ, Beaumont J, Elamin F et al (2022) Method of Micro-Sampling Human Dentine Collagen for Stable Isotope Analysis. Rapid Communications in Mass Spectrometry. 36(13): e9305.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18875
dc.descriptionYesen_US
dc.description.abstractSampling of dentine for stable carbon (δ13 C) and nitrogen (δ15 N) isotope ratios in the direction of tooth growth allows the study of temporal changes to the diet and physiological stress of an individual during tooth formation. Current methods of sampling permanent teeth using 1mm increments provide temporal resolution of six - nine months at best depending on the tooth chosen. While this gives sufficient sample sizes for reliable analysis by mass spectrometry, sectioning the dentine across the incremental structures results in a rolling average of the isotope ratios. A novel method of incremental dentine collagen sampling has been developed to decrease the collagen increment size to 0.35mm along the incremental structures thus reducing averaging and improving the temporal resolution of short-term changes within the δ13 C and δ15 N values. This study presents data for a MicroMill-assisted sampling method that allows for sampling at 0.35mm width x 1mm depth increments following the incremental growth pattern of dentine. A NewWave MicroMill was used to sample the demineralised dentine section of modern donated human third molars from Sudan and compared to data from the same teeth using the 1mm incremental sectioning method 2 from Beaumont et al. (2013). The δ13 C and δ15 N isotopic data showed an increased temporal resolution, with each increment providing data for two-four months of dentine formation. The data show the potential of this method for studying dietary reconstruction, nutritional stress, and physiological change with greater temporal resolution potentially to seasonal level and with less attenuation of the δ13 C and δ15 N values than was previously possible from human dentine.en_US
dc.language.isoenen_US
dc.rights© 2022 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectMicro-samplingen_US
dc.subjectHuman dentine collagenen_US
dc.subjectStable carbon isotopesen_US
dc.subjectStable nitrogen isotopesen_US
dc.subjectTooth formationen_US
dc.subjectTemporal changesen_US
dc.subjectDieten_US
dc.titleMethod of Micro-Sampling Human Dentine Collagen for Stable Isotope Analysisen_US
dc.status.refereedYesen_US
dc.date.application2022-03-31
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.1002/rcm.9305
dc.rights.licenseCC-BYen_US
dc.date.updated2022-04-12T13:43:53Z
refterms.dateFOA2022-04-13T07:37:47Z
dc.openaccess.statusopenAccessen_US
dc.OrcidIdhttps://orcid.org/https://doi.org/10.1002/rcm.9305en_US


Item file(s)

Thumbnail
Name:
curtis_et_al_2022.pdf
Size:
1.755Mb
Format:
PDF
Thumbnail
Name:
curtis_et_al_2022.pdf
Size:
1.370Mb
Format:
PDF
Description:
Keep suppressed - AAM

This item appears in the following Collection(s)

Show simple item record