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    Method of Micro-Sampling Human Dentine Collagen for Stable Isotope Analysis

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    Publication date
    2022-07-15
    Author
    Curtis, Mandi J.
    Beaumont, Julia
    Elamin, F.
    Wilson, Andrew S.
    Koon, Hannah E.C.
    Keyword
    Micro-sampling
    Human dentine collagen
    Stable carbon isotopes
    Stable nitrogen isotopes
    Tooth formation
    Temporal changes
    Diet
    Rights
    © 2022 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    Open Access status
    openAccess
    
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    Abstract
    Sampling of dentine for stable carbon (δ13 C) and nitrogen (δ15 N) isotope ratios in the direction of tooth growth allows the study of temporal changes to the diet and physiological stress of an individual during tooth formation. Current methods of sampling permanent teeth using 1mm increments provide temporal resolution of six - nine months at best depending on the tooth chosen. While this gives sufficient sample sizes for reliable analysis by mass spectrometry, sectioning the dentine across the incremental structures results in a rolling average of the isotope ratios. A novel method of incremental dentine collagen sampling has been developed to decrease the collagen increment size to 0.35mm along the incremental structures thus reducing averaging and improving the temporal resolution of short-term changes within the δ13 C and δ15 N values. This study presents data for a MicroMill-assisted sampling method that allows for sampling at 0.35mm width x 1mm depth increments following the incremental growth pattern of dentine. A NewWave MicroMill was used to sample the demineralised dentine section of modern donated human third molars from Sudan and compared to data from the same teeth using the 1mm incremental sectioning method 2 from Beaumont et al. (2013). The δ13 C and δ15 N isotopic data showed an increased temporal resolution, with each increment providing data for two-four months of dentine formation. The data show the potential of this method for studying dietary reconstruction, nutritional stress, and physiological change with greater temporal resolution potentially to seasonal level and with less attenuation of the δ13 C and δ15 N values than was previously possible from human dentine.
    URI
    http://hdl.handle.net/10454/18875
    Version
    Published version
    Citation
    Curtis MJ, Beaumont J, Elamin F et al (2022) Method of Micro-Sampling Human Dentine Collagen for Stable Isotope Analysis. Rapid Communications in Mass Spectrometry. 36(13): e9305.
    Link to publisher’s version
    https://doi.org/10.1002/rcm.9305
    Type
    Article
    Collections
    Health Studies Publications

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