BRADFORD SCHOLARS

    • Sign in
    View Item 
    •   Bradford Scholars
    • University of Bradford eTheses
    • Theses
    • View Item
    •   Bradford Scholars
    • University of Bradford eTheses
    • Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Bradford ScholarsCommunitiesAuthorsTitlesSubjectsPublication DateThis CollectionAuthorsTitlesSubjectsPublication Date

    My Account

    Sign in

    HELP

    Bradford Scholars FAQsCopyright Fact SheetPolicies Fact SheetDeposit Terms and ConditionsDigital Preservation Policy

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Surface engineering, characterisation and applications of synthetic polymers for tissue engineering and regenerative medicine. Investigation of the response of MG63 osteosarcoma cell line to modified surface topographies, mechanical properties and cell-surface interactions using different synthetic polymers fabricated in house with various topographical features

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    View/Open
    PhD Thesis (5.945Mb)
    Download
    Publication date
    2019
    Author
    Rehman, Ramisha U.
    Supervisor
    Youseffi, Mansour
    Sefat, Farshid
    Katsikogianni, Maria G.
    Keyword
    Surface topography
    Surface energy
    Mechanical properties
    Chemistry
    Cell adhesion
    Cell morphology
    Tissue engineering
    Biomaterials
    Regenerative medicine
    Rights
    Creative Commons License
    The University of Bradford theses are licenced under a Creative Commons Licence.
    Institution
    University of Bradford
    Department
    Biomedical and Electronics Engineering Department, Faculty of Engineering and Informatics
    Awarded
    2019
    
    Metadata
    Show full item record
    Abstract
    At present there is an extraordinary need to overcome barriers in regards to discovering novel and enhanced biomaterials for various tissue engineering applications. The need for durable orthopaedic implants is on the rise to limit issues such as revision surgery. A promising pathway to enhance fixation is to accelerate the onset and rate of early cellular adhesion and bone growth through nanoscale surface topography at the implant surface. The main aim of this research project was to investigate cellular response to altered physical and mechanical characteristics of materials suitable for orthopaedic applications. Four injection moulded polymeric substrates were produced, each with varied compositional and topographical characteristics. The four materials fabricated are Polyether-ether-ketone (PEEK), PEEK with 30% glass fibre (GL/PEEK) composite, PEEK and GL/PEEK with grooved topography. SEM and AFM analysis was used to investigate the groove dimensions and surface roughness of all samples followed by mechanical testing using a nano indenter to detect the Young’s modulus, stiffness and hardness of all four substrates. These tests were performed to determine which material has similar characteristics to cortical bone. These tests were followed by wettability and surface energy testing. Cell-substrate adhesion was examined using a cell viability assay to identify if there is a significant difference (p<0.05) between the percentage of viable cells on all four PEEK based materials. Imaging of MG-63 osteosarcoma cells using immunohistochemistry staining kits was conducted to observe the relationship between cell length and surface topography followed by a comparison between HaCaT (skin) cells and MG-63 (bone) cells. Following experimental testing mechanical variations between PEEK and GL/PEEK were identified alongside physical characterization differences. The grooved topography increased the surface roughness of PEEK and GL/PEEK in comparison to the planar surface. After 72 hours a correlation between the increased surface roughness and the percentage of viable MG-63 cells could be identified. When assessing the effect surface topography has on the water contact angles and surface energy, all four substrates showed no correlation. However, the grooved topography did increase the water contact angle and reduced the surface energy of PEEK in comparison to planar PEEK. Images of the four substrates after cell culture observed the grooved topography to affect the cellular orientation of both MG-63 and HaCaT cells. Polycaprolactone (PCL) scaffolds with a concentration of 1, 3, and 5% triclosan (an antimicrobial and antifungal agent) were fabricated using electrospinning. In addition to PCL + Triclosan scaffolds PCL with a concentration of 1% silver (an antimicrobial agent that can reduce the risk of infection) and 1, 3, and 5% triclosan were also electrospun. The pore size and fibre diameters of the scaffolds were investigated using SEM and Image J software followed by wettability and surface energy testing. MG-63 cells were cultured on all PCL scaffolds to study cellular viability percentage after 24 and 72 hours. The findings obtained showed the physical characteristics of PCL scaffolds to affect cellular viability of MG-63 cells. The output from these findings aim to provide data at a proof of concept level in understanding the relationship between the mechanical and physical characteristics of biomaterials and cellular behaviour.
    URI
    http://hdl.handle.net/10454/18671
    Type
    Thesis
    Qualification name
    PhD
    Collections
    Theses

    entitlement

     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Polymer Technologies for the Control of Bacterial Adhesion - From Fundamental to Applied Science and Technology

      Katsikogianni, Maria G.; Missirlis, Y.F. (2014)
      This article describes how an insight into the chemical and physical cues that affect bacterial adhesion and biofilm formation can provide ideas for creating successful antifouling or antimicrobial surfaces. To facilitate the design of new materials, the role of physical and chemical properties on bacterial adhesion is reviewed. The current approaches to reduce bacterial adhesion to various polymeric surfaces are discussed, as well as how multidisciplinary research on surface design and engineering may have an impact on both fundamental and applied microbiological science and technology.
    • Thumbnail

      On Bezier surfaces in three-dimensional Minkowski space

      Ugail, Hassan; Marquez, M.C.; Yilmaz, A. (2011)
      In this paper, we study Bézier surfaces in View the MathML source three-dimensional Minkowski space. In particular, we focus on timelike and spacelike cases for Bézier surfaces. We also deal with the Plateau¿Bézier problem in View the MathML source, obtaining conditions over the control net to be extremal of the Dirichlet function for both timelike and spacelike Bézier surfaces. Moreover, we provide interesting examples showing the behavior of the Plateau¿Bézier problem in View the MathML source and illustrating the relationship between it and the corresponding Plateau¿Bézier problem in the Euclidean space R3.
    • Thumbnail

      Biomaterial Functionalized Surfaces for Reducing Bacterial Adhesion and Infection

      Katsikogianni, Maria G.; Wood, David J.; Missirlis, Y.F. (2016)
      This chapter describes the current approaches to reduce bacterial adhesion to various biomaterial surfaces, focusing on nonfouling surfaces through patterning and hydrophobicity plasma-assisted surface treatment and deposition; incorporation of antimicrobials, antibiotics, antibiofilms, and natural extracts that are either immobilized or released; dual function antimicrobial surfaces; incorporation of nonpathogenic bacteria, bacteriophages, and biofilm dispersal agents but also reduced bacterial adhesion through tissue integration. To facilitate the design of new materials, the role of physical, chemical, and biological surface properties on bacterial adhesion is reviewed in each case, as an insight into the chemical and physical cues that affect bacterial adhesion and biofilm formation can provide ideas for creating successful antifouling or antimicrobial surfaces. The application of these surfaces is explored based on the clinical needs and the market gaps. How multidisciplinary research on surface design and engineering may have an impact on both fundamental understanding of bacterial adhesion to biomaterials and applied biomaterial science and technology is finally discussed.
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.