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dc.contributor.advisorPalmer, Timothy M.
dc.contributor.advisorWilliams, Jamie J.L.
dc.contributor.advisorNasim, Md. Talat
dc.contributor.advisorElies, Jacobo
dc.contributor.authorDurham, Gillian A.
dc.date.accessioned2021-12-01T12:18:25Z
dc.date.available2021-12-01T12:18:25Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/10454/18665
dc.description.abstractPulmonary arterial hypertension (PAH) is a rare, devastating disease with no cure. Current treatment consists of a cocktail of vasodilators which relieve symptoms of PAH but do not treat the cause. Thus, there is a need for novel drugs that target the underlying pathological causes of PAH. PAH is a multi-factorial, but one key contributor is the pro-inflammatory cytokine IL-6 which stimulates pro-inflammatory and pro-angiogenic signalling mediated by the JAK/STAT pathway. One way in which IL-6 signalling via JAK/STAT is inhibited is via SOCS3 in a type of negative feedback loop whereby IL-6 induces transcription of SOCS3, which then attenuates further JAK/STAT signalling. SOCS3 can also be induced by cAMP. This is interesting as prostanoids, a type of drug used in the treatment of PAH due to its vasodilator effects and the only type to show any efficacy improving the life expectancy of PAH patients, acts by mobilising cAMP. Thus, prostanoid stimulation of cAMP could potentially limit IL-6 signalling via the induction of SOCS3. This is a novel mechanism of prostanoids which has not previously been considered. This study investigated the capability of prostanoids to limit the pro-inflammatory/pro-angiogenic effects of IL-6 that enable PAH to develop. Initial experiments confirmed that vascular endothelial cells responded to prostanoids which increased SOCS3 and limited IL-6 signalling activity. Further experiments utilising SOCS3 KO endothelial cell models demonstrated prostanoid inhibition of IL-6 signalling was due in part to SOCS3. In conclusion, this project has confirmed that prostanoids do limit the pro-inflammatory effects induced by IL-6 and that this is in part due to SOCS3. Although the exact mechanism is yet to be discovered, it will be beneficial in the treatment of PAH as it provides currently unexploited drug targets which can be considered for future PAH therapies.en_US
dc.description.sponsorshipBritish Heart Foundationen_US
dc.language.isoenen_US
dc.rights<a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>.eng
dc.subjectPulmonary arterial hypertensionen_US
dc.subjectIL-6en_US
dc.subjectJAK/STAT signallingen_US
dc.subjectEndothelial cellsen_US
dc.subjectProstanoidsen_US
dc.subjectSOCS3 (Suppressor Of Cytokine Signaling 3)en_US
dc.subjectDrug therapiesen_US
dc.titleProstanoid-mediated Inhibition of IL-6 Trans-Signalling in Pulmonary Arterial Hypertension: a Role for Suppressor of Cytokine Signalling 3?en_US
dc.type.qualificationleveldoctoralen_US
dc.publisher.institutionUniversity of Bradfordeng
dc.publisher.departmentSchool of Pharmacy and Medical Sciences Faculty of Life Sciencesen_US
dc.typeThesiseng
dc.type.qualificationnamePhDen_US
dc.date.awarded2019
refterms.dateFOA2021-12-01T12:18:25Z


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