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dc.contributor.authorHolland, J.D.R.
dc.contributor.authorWebster, G.
dc.contributor.authorRooney, P.
dc.contributor.authorWilshaw, Stacy-Paul
dc.contributor.authorJennings, L.M.
dc.contributor.authorBerry, H.E.
dc.date.accessioned2021-06-29T20:57:51Z
dc.date.accessioned2021-07-14T10:19:20Z
dc.date.available2021-06-29T20:57:51Z
dc.date.available2021-07-14T10:19:20Z
dc.date.issued2021-06
dc.identifier.citationHolland JDR, Webster G, Rooney P, Wilshaw S-P, Jennings LM and Berry HE (2021) Effects of Chemical and Radiation Sterilisation on the Biological and Biomechanical Properties of Decellularised Porcine Peripheral Nerves. Frontiers In Bioengineering And Biotechnology. 9: 660453.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18543
dc.descriptionyesen_US
dc.description.abstractThere is a clinical need for novel graft materials for the repair of peripheral nerve defects. A decellularisation process has been developed for porcine peripheral nerves, yielding a material with potentially significant advantages over other devices currently being used clinically (such as autografts and nerve guidance conduits). Grafts derived from xenogeneic tissues should undergo sterilisation prior to clinical use. It has been reported that sterilisation methods may adversely affect the properties of decellularised tissues, and therefore potentially negatively impact on the ability to promote tissue regeneration. In this study, decellularised nerves were produced and sterilised by treatment with 0.1% (v/v) PAA, gamma radiation (25-28 kGy) or E Beam (33-37 kGy). The effect of sterilisation on the decellularised nerves was determined by cytotoxicity testing, histological staining, hydroxyproline assays, uniaxial tensile testing, antibody labelling for collagen type IV, laminin and fibronectin in the basal lamina, and differential scanning calorimetry. This study concluded that decellularised nerves retained biocompatibility following sterilisation. However, sterilisation affected the mechanical properties (PAA, gamma radiation), endoneurial structure and basement membrane composition (PAA) of decellularised nerves. No such alterations were observed following E Beam treatment, suggesting that this method may be preferable for the sterilisation of decellularised porcine peripheral nerves.en_US
dc.language.isoenen_US
dc.rights© 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply. with these terms.en_US
dc.subjectPeripheral nervesen_US
dc.subjectPorcine peripheral nervesen_US
dc.subjectDecellularisationen_US
dc.subjectSterilisationen_US
dc.subjectCollagen type IVen_US
dc.subjectBiomechanical propertiesen_US
dc.subjectRadiation sterilisationen_US
dc.subjectE Beam sterilisationen_US
dc.titleEffects of Chemical and Radiation Sterilisation on the Biological and Biomechanical Properties of Decellularised Porcine Peripheral Nervesen_US
dc.status.refereedyesen_US
dc.date.Accepted2021-05-10
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.3389/fbioe.2021.660453
dc.date.updated2021-06-29T19:58:01Z
refterms.dateFOA2021-07-14T10:19:55Z
dc.openaccess.statusGolden_US


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