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dc.contributor.authorMardaryev, Andrei N.
dc.contributor.authorFessing, Michael Y.
dc.date.accessioned2021-05-25T10:25:40Z
dc.date.accessioned2021-06-11T09:45:59Z
dc.date.available2021-05-25T10:25:40Z
dc.date.available2021-06-11T09:45:59Z
dc.date.issued2020-04
dc.identifier.citationMardaryev AN and Fessing MY (2020) 3D-FISH analysis of the spatial genome organization in skin cells in situ. In: Botchkareva NV and Westgate GE (Eds.) Molecular Dermatology. Methods in Molecular Biology, vol 2154. Humana, New York. 217-230.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18511
dc.descriptionNoen_US
dc.description.abstractSpatial genome organization in the cell nucleus plays a crucial role in the control of genome functions. Our knowledge about spatial genome organization is relying on the advances in gene imaging technologies and the biochemical approaches based on the spatial dependent ligation of the genomic regions. Fluorescent in situ hybridization using specific fluorescent DNA and RNA probes in cells and tissues with the spatially preserved nuclear and genome architecture (3D-FISH) provides a powerful tool for the further advancement of our knowledge about genome structure and functions. Here we describe the 3D-FISH protocols allowing for such an analysis in mammalian tissue in situ including in the skin. These protocols include DNA probe amplification and labeling; tissue fixation; preservation and preparation for hybridization; hybridization of the DNA probes with genomic DNA in the tissue; and post-hybridization tissue sample processing.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1007/978-1-0716-0648-3_18en_US
dc.subjectEpigeneticsen_US
dc.subjectSpatial genome organizationen_US
dc.subjectFluorescent in situ hybridizationen_US
dc.subject3D FISH analysisen_US
dc.title3D-FISH analysis of the spatial genome organization in skin cells in situen_US
dc.status.refereedYesen_US
dc.date.Accepted2020
dc.date.application2020-04-21
dc.typeArticleen_US
dc.type.versionNo full-text in the repositoryen_US
dc.date.updated2021-05-25T09:25:46Z
dc.openaccess.statusNot Open Accessen_US


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