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dc.contributor.authorSchulze, H.
dc.contributor.authorWilson, H.
dc.contributor.authorCara, I.
dc.contributor.authorCarter, Steven
dc.contributor.authorDyson, Edward N.
dc.contributor.authorElangovan, R.
dc.contributor.authorRimmer, Stephen
dc.contributor.authorBachmann, T.T.
dc.date.accessioned2021-05-12T12:03:53Z
dc.date.accessioned2021-05-26T08:45:16Z
dc.date.available2021-05-12T12:03:53Z
dc.date.available2021-05-26T08:45:16Z
dc.date.issued2021-03-08
dc.identifier.citationSchulze H, Wilson H, Cara I et al (2021) Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers. Sensors (Basel, Switzerland). 21(5): 1872-1884.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18494
dc.descriptionYesen_US
dc.description.abstractRapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.en_US
dc.language.isoenen_US
dc.publisherMDPI
dc.relation.isreferencedbyhttps://doi.org/10.3390/s21051872en_US
dc.rights(c) 2021 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (https://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectEISen_US
dc.subjectHighly branched polymersen_US
dc.subjectBacteria pathogen detectionen_US
dc.subjectLabel-freeen_US
dc.subjectPoint of care diagnosticsen_US
dc.subjectAMRen_US
dc.titleLabel-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymersen_US
dc.status.refereedYesen_US
dc.date.Accepted2021-03-05
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2021-05-12T11:04:04Z
refterms.dateFOA2021-05-26T08:45:37Z
dc.openaccess.statusGolden_US


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