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dc.contributor.authorMatoug-Elwerfelli, M.
dc.contributor.authorNazzal, H.
dc.contributor.authorRaif, E.M.
dc.contributor.authorWilshaw, Stacy-Paul
dc.contributor.authorEsteves, F.
dc.contributor.authorDuggal, M.
dc.date.accessioned2021-02-23T07:25:12Z
dc.date.accessioned2021-02-26T15:17:38Z
dc.date.available2021-02-23T07:25:12Z
dc.date.available2021-02-26T15:17:38Z
dc.date.issued2020-12
dc.identifier.citationMatoug-Elwerfelli M, Nazzal H, Raif EM et al (2020) Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix. Scientific Reports. 10(1): 21553.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18375
dc.descriptionYesen_US
dc.description.abstractImplementing the principles of tissue engineering within the clinical management of non-vital immature permanent teeth is of clinical interest. However, the ideal scaffold remains elusive. The aim of this work was to assess the feasibility of decellularising rat dental pulp tissue and evaluate the ability of such scaffold to support stem cell repopulation. Rat dental pulps were retrieved and divided into control and decellularised groups. The decellularisation protocol incorporated a low detergent concentration and hypotonic buffers. After decellularisation, the scaffolds were characterised histologically, immunohistochemistry and the residual DNA content quantified. Surface topography was also viewed under scanning electron microscopy. Biocompatibility was evaluated using cytotoxicity assays utilising L-929 cell line. Decellularised scaffolds were recellularised with human dental pulp stem cells up to 14 days in vitro. Cellular viability was assessed using LIVE/DEAD stain kit and the recellularised scaffolds were further assessed histologically and immunolabelled using makers for odontoblastic differentiation, cytoskeleton components and growth factors. Analysis of the decellularised scaffolds revealed an acellular matrix with histological preservation of structural components. Decellularised scaffolds were biocompatible and able to support stem cell survival following recellularisation. Immunolabelling of the recellularised scaffolds demonstrated positive cellular expression against the tested markers in culture. This study has demonstrated the feasibility of developing a biocompatible decellularised dental pulp scaffold, which is able to support dental pulp stem cell repopulation. Clinically, decellularised pulp tissue could possibly be a suitable scaffold for use within regenerative (reparative) endodontic techniques.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1038/s41598-020-78477-xen_US
dc.rights(c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectTissue engineeringen_US
dc.subjectRat dental pulpen_US
dc.subjectStem cell repopulationen_US
dc.titleEx-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrixen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-11-18
dc.date.application2020-12-09
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2021-02-23T07:25:27Z
refterms.dateFOA2021-02-26T15:19:58Z
dc.openaccess.statusGolden_US


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