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dc.contributor.authorSwift, Thomas
dc.contributor.authorCaseley, Emily
dc.contributor.authorPinnock, A.
dc.contributor.authorShepherd, J.
dc.contributor.authorShivshetty, N.
dc.contributor.authorGarg, P.
dc.contributor.authorIan Douglas, C.W.
dc.contributor.authorMacNeil, S.
dc.contributor.authorRimmer, Stephen
dc.date.accessioned2021-02-22T18:48:14Z
dc.date.accessioned2021-02-26T14:45:20Z
dc.date.available2021-02-22T18:48:14Z
dc.date.available2021-02-26T14:45:20Z
dc.date.issued2021-01-27
dc.identifier.citationSwift T, Caseley E, Pinnock A et al (2021) Branched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymer. Royal Society Open Science. 8(1): 201655.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18373
dc.descriptionYesen_US
dc.description.abstractBranched poly(N-isopropylacrylamide) was functionalized with Amphotericin B (AmB) at the chain ends to produce an antifungal material. The polymer showed antifungal properties against AmB-sensitive strains of Candida albicans, Fusarium keratoplasticum and Aspergillus flavus (minimal inhibitory concentration ranged from 5 to 500 µg ml−1) but was not effective against an AmB resistant strain of C. albicans nor against Candida tropicalis. The polymer end groups bound to the AmB target, ergosterol, and the fluorescence spectrum of a dye used as a solvatochromic probe, Nile red, was blue shifted indicating that segments of the polymer became desolvated on binding. The polymer was less toxic to corneal and renal epithelial cells and explanted corneal tissue than the free drug. Also, the polymer did not induce reactive oxygen species release from peripheral blood mononuclear cells, nor did it cause a substantial release of the proinflammatory cytokines, tumour necrosis factor-α and interleukin-1β (at 0.5 mg ml−1).en_US
dc.description.sponsorshipWelcome Trust DBT Alliance (0998800/B/12/Z) and MRC (UK) (grant no. 16038)en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1098/rsos.201655en_US
dc.rights(c) 2021 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectSmart polymersen_US
dc.subjectBranched polymeren_US
dc.subjectFungien_US
dc.titleBranched amphotericin functional poly(N-isopropyl acrylamide): an antifungal polymeren_US
dc.status.refereedYesen_US
dc.date.Accepted2020-11-20
dc.date.application2021-01-13
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.date.updated2021-02-22T18:48:24Z
refterms.dateFOA2021-02-26T14:46:55Z
dc.openaccess.statusGolden_US


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