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dc.contributor.authorArabpour, Z.
dc.contributor.authorBaradaran-Rafii, A.
dc.contributor.authorBakhshaiesh, N.L.
dc.contributor.authorAi, J.
dc.contributor.authorEbrahimi-Barough, S.
dc.contributor.authorMalekabadi, H.E.
dc.contributor.authorNazeri, N.
dc.contributor.authorVaez, A.
dc.contributor.authorSalehi, M.
dc.contributor.authorSefat, Farshid
dc.contributor.authorOstad, S.N.
dc.date.accessioned2021-02-21T17:08:40Z
dc.date.accessioned2021-02-25T10:33:20Z
dc.date.available2021-02-21T17:08:40Z
dc.date.available2021-02-25T10:33:20Z
dc.date.issued2019-10
dc.identifier.citationArabpour Z, Baradaran-Rafii A, Bakhshaiesh NL et al (2019) Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications. Journal of Biomedical Materials Research - Part A. 107(10): 2340-2349.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18371
dc.descriptionYesen_US
dc.description.abstractTissue engineering is one of the most promising areas for treatment of various ophthalmic diseases particularly for patients who suffer from limbal stem cell deficiency and this is due to the lack of existence of appropriate matrix for stem cell regeneration. The aim of this research project is to design and fabricate triple layered electrospun nanofibers as a suitable corneal tissue engineering scaffold and the objective is to investigate and perform various in vitro tests to find the most optimum and suitable scaffold for this purpose. Electrospun scaffolds were prepared in three layers. Poly(d, l-lactide-co-glycolide; PLGA, 50:50) nanofibers were electrospun as outer and inner layers of the scaffold and aligned type I collagen nanofibers were electrospun in the middle layer. Furthermore, the scaffolds were cross-linked by 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride and glutaraldehyde. Structural, physical, and mechanical properties of scaffolds were investigated by using N2 adsorption/desorption isotherms, Fourier transform infrared spectroscopy, contact angle measurement, tensile test, degradation, shrinkage analysis, and scanning electron microscopy (SEM). In addition, capability to support cell attachment and viability were characterized by SEM, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, and 4′,6-diamidino-2-phenylindole staining. According to the result of Brunauer–Emmett–Teller analysis, specific surface area of electrospun scaffold was about 23.7 m2 g-1. Tensile tests on cross-linked scaffolds represented more suitable hydrophilicity and tensile behavior. In addition, degradation rate analysis indicated that noncross-linked scaffolds degraded faster than cross-linked one and cross-linking led to controlled shrinkage in the scaffold. The SEM analysis depicted nano-sized fibers in good shape. Also, the in vitro study represented an improved cell attachment and proliferation in the presence of human endometrial stem cells for both cross-linked and noncross-linked samples. The current study suggests the possibility of producing an appropriate substrate for successful cornea tissue engineering with a novel design.en_US
dc.description.sponsorshipDeputy of Research, Tehran University of Medical Science. Grant Number: 93‐01‐33‐25613en_US
dc.language.isoenen_US
dc.rights© 2019 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Arabpour Z, Baradaran-Rafii A, Bakhshaiesh NL et al (2019) Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications. Journal of Biomedical Materials Research - Part A. 107(10): 2340-2349, which has been published in final form at https://doi.org/10.1002/jbm.a.36742. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
dc.subjectCollagenen_US
dc.subjectCornealen_US
dc.subjectElectrospinningen_US
dc.subjectHuman endometrial stem cells (hEnSCs)en_US
dc.subjectPLGAen_US
dc.subjectTissue engineeringen_US
dc.titleDesign and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applicationsen_US
dc.status.refereedYesen_US
dc.date.Accepted2019-05-30
dc.date.application2019-06-03
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.identifier.doihttps://doi.org/10.1002/jbm.a.36742
dc.date.updated2021-02-21T17:08:45Z
refterms.dateFOA2021-02-25T10:40:15Z
dc.openaccess.statusGreenen_US


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