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    Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications

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    Publication date
    2019-10
    Author
    Arabpour, Z.
    Baradaran-Rafii, A.
    Bakhshaiesh, N.L.
    Ai, J.
    Ebrahimi-Barough, S.
    Malekabadi, H.E.
    Nazeri, N.
    Vaez, A.
    Salehi, M.
    Sefat, Farshid
    Ostad, S.N.
    Show allShow less
    Keyword
    Collagen
    Corneal
    Electrospinning
    Human endometrial stem cells (hEnSCs)
    PLGA
    Tissue engineering
    Rights
    © 2019 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Arabpour Z, Baradaran-Rafii A, Bakhshaiesh NL et al (2019) Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications. Journal of Biomedical Materials Research - Part A. 107(10): 2340-2349, which has been published in final form at https://doi.org/10.1002/jbm.a.36742. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
    Peer-Reviewed
    Yes
    Open Access status
    Green
    
    Metadata
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    Abstract
    Tissue engineering is one of the most promising areas for treatment of various ophthalmic diseases particularly for patients who suffer from limbal stem cell deficiency and this is due to the lack of existence of appropriate matrix for stem cell regeneration. The aim of this research project is to design and fabricate triple layered electrospun nanofibers as a suitable corneal tissue engineering scaffold and the objective is to investigate and perform various in vitro tests to find the most optimum and suitable scaffold for this purpose. Electrospun scaffolds were prepared in three layers. Poly(d, l-lactide-co-glycolide; PLGA, 50:50) nanofibers were electrospun as outer and inner layers of the scaffold and aligned type I collagen nanofibers were electrospun in the middle layer. Furthermore, the scaffolds were cross-linked by 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride and glutaraldehyde. Structural, physical, and mechanical properties of scaffolds were investigated by using N2 adsorption/desorption isotherms, Fourier transform infrared spectroscopy, contact angle measurement, tensile test, degradation, shrinkage analysis, and scanning electron microscopy (SEM). In addition, capability to support cell attachment and viability were characterized by SEM, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, and 4′,6-diamidino-2-phenylindole staining. According to the result of Brunauer–Emmett–Teller analysis, specific surface area of electrospun scaffold was about 23.7 m2 g-1. Tensile tests on cross-linked scaffolds represented more suitable hydrophilicity and tensile behavior. In addition, degradation rate analysis indicated that noncross-linked scaffolds degraded faster than cross-linked one and cross-linking led to controlled shrinkage in the scaffold. The SEM analysis depicted nano-sized fibers in good shape. Also, the in vitro study represented an improved cell attachment and proliferation in the presence of human endometrial stem cells for both cross-linked and noncross-linked samples. The current study suggests the possibility of producing an appropriate substrate for successful cornea tissue engineering with a novel design.
    URI
    http://hdl.handle.net/10454/18371
    Version
    Accepted manuscript
    Citation
    Arabpour Z, Baradaran-Rafii A, Bakhshaiesh NL et al (2019) Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications. Journal of Biomedical Materials Research - Part A. 107(10): 2340-2349.
    Link to publisher’s version
    https://doi.org/10.1002/jbm.a.36742
    Type
    Article
    Collections
    Engineering and Digital Technology Publications

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