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dc.contributor.authorAghmiuni, A.I.
dc.contributor.authorHeidari Keshel, S.
dc.contributor.authorSefat, Farshid
dc.contributor.authorAkbarzadehKhiyavi, A.
dc.date.accessioned2021-02-21T16:29:32Z
dc.date.accessioned2021-02-25T08:47:39Z
dc.date.available2021-02-21T16:29:32Z
dc.date.available2021-02-25T08:47:39Z
dc.date.issued2021-01
dc.identifier.citationAghmiuni AI, Heidari Keshel S, Sefat F et al (2021) Fabrication of 3D hybrid scaffold by combination technique of electrospinning-like and freeze-drying to create mechanotransduction signals and mimic extracellular matrix function of skin. Materials Science and Engineering C. 120: 111752.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18370
dc.descriptionYesen_US
dc.description.abstractFabrication of extracellular matrix (ECM)-like scaffolds (in terms of structural-functional) is the main challenge in skin tissue engineering. Herein, inspired by macromolecular components of ECM, a novel hybrid scaffold suggested which includes silk/hyaluronan (SF/HA) bio-complex modified by PCP: [polyethylene glycol/chitosan/poly(ɛ-caprolactone)] copolymer containing collagen to differentiate human-adipose-derived stem cells into keratinocytes. In followed by, different weight ratios (wt%) of SF/HA (S1:100/0, S2:80/20, S3:50/50) were applied to study the role of SF/HA in the improvement of physicochemical and biological functions of scaffolds. Notably, the combination of electrospinning-like and freeze-drying methods was also utilized as a new method to create a coherent 3D-network. The results indicated this novel technique was led to ~8% improvement of the scaffold's ductility and ~17% decrease in mean pore diameter, compared to the freeze-drying method. Moreover, the increase of HA (>20wt%) increased porosity to 99%, however, higher tensile strength, modulus, and water absorption% were related to S2 (38.1, 0.32 MPa, 75.3%). More expression of keratinocytes along with growth pattern similar to skin was also observed on S2. This study showed control of HA content creates a microporous-environment with proper modulus and swelling%, although, the role of collagen/PCP as base biocomposite and fabrication technique was undeniable on the inductive signaling of cells. Such a scaffold can mimic skin properties and act as the growth factor through inducing keratinocytes differentiation.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.msec.2020.111752en_US
dc.rights(c) 2021 Crown Copyright. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (https://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.subjectComposite scaffoldsen_US
dc.subjectECM componentsen_US
dc.subjectSkin tissue engineeringen_US
dc.subjectFabrication techniquesen_US
dc.subjectKeratinocytesen_US
dc.titleFabrication of 3D hybrid scaffold by combination technique of electrospinning-like and freeze-drying to create mechanotransduction signals and mimic extracellular matrix function of skinen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-11-21
dc.date.application2020-11-27
dc.typeArticleen_US
dc.date.EndofEmbargo2021-11-27
dc.type.versionAccepted manuscripten_US
dc.description.publicnotesThe full-text of this article will be released for public view at the end of the publisher embargo on 27 Nov 2021.
dc.date.updated2021-02-21T16:29:45Z
refterms.dateFOA2021-02-25T08:52:03Z
dc.openaccess.statusGreenen_US


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