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dc.contributor.authorGuo, Xiaoxiao
dc.contributor.authorElkashef, Sara M.
dc.contributor.authorPatel, Anjana
dc.contributor.authorRibeiro Morais, Goreti
dc.contributor.authorShnyder, Steven D.
dc.contributor.authorLoadman, Paul M.
dc.contributor.authorPatterson, Laurence H.
dc.contributor.authorFalconer, Robert A.
dc.date.accessioned2021-02-15T11:07:06Z
dc.date.accessioned2021-02-16T15:05:05Z
dc.date.available2021-02-15T11:07:06Z
dc.date.available2021-02-16T15:05:05Z
dc.date.issued2021-05-01
dc.identifier.citationGuo X, Elkashef SM, Patel A et al (2021) An assay for quantitative analysis of polysialic acid expression in cancer cells. Carbohydrate Polymers. 259: 117741.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18344
dc.descriptionYesen_US
dc.description.abstractPolysialic acid (polySia) is a linear polysaccharide comprised of N-acetylneuraminic acid residues and its over-expression in cancer cells has been correlated with poor clinical prognosis. An assay has been developed for quantitative analysis of cellular polySia expression. This was achieved by extracting and purifying released polySia from glycoproteins by mild acid hydrolysis and optimised organic extraction. The polySia was further hydrolysed into Sia monomers, followed by fluorescent labelling and quantitative analysis. The assay was qualified utilising endoneuraminidase-NF to remove polySia from the surface of C6-ST8SiaII cancer cells (EC50 = 2.13 ng/ml). The result was comparable to that obtained in a polySia-specific cellular ELISA assay. Furthermore, the assay proved suitable for evaluation of changes in polySia expression following treatment with a small molecule inhibitor of polysialylation. Given the importance of polySia in multiple disease states, notably cancer, this is a potentially vital tool with applications in the fields of drug discovery and glycobiology.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.carbpol.2021.117741en_US
dc.rights© 2021 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (https://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.subjectPolysialic aciden_US
dc.subjectSialic aciden_US
dc.subjectQuantitative analysisen_US
dc.subjectCell-based methoden_US
dc.subjectInhibitor screeningen_US
dc.titleAn assay for quantitative analysis of polysialic acid expression in cancer cellsen_US
dc.status.refereedYesen_US
dc.date.Accepted2021-01-27
dc.date.application2021-02-02
dc.typeArticleen_US
dc.date.EndofEmbargo2022-02-03
dc.type.versionAccepted manuscripten_US
dc.description.publicnotesThe full-text of this article will be released for public view at the end of the publisher embargo on 3 Feb 2022.en_US
dc.date.updated2021-02-15T11:07:07Z
refterms.dateFOA2021-02-17T10:02:19Z
dc.openaccess.statusGreenen_US


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