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dc.contributor.authorHutin, D.
dc.contributor.authorGrimaldi, Giulia
dc.contributor.authorMatthews, J.
dc.date.accessioned2021-02-10T15:04:22Z
dc.date.available2021-02-10T15:04:22Z
dc.date.issued2018
dc.identifier.citationHutin D, Grimaldi G and Matthews J (2018) Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity. In: Chang P (ed) ADP-ribosylation and NAD+ utilizing enzymes. Methods in Molecular Biology: 1813. Humana: New York.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18336
dc.descriptionNoen_US
dc.description.abstractTCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1007/978-1-4939-8588-3_8en_US
dc.subjectImmunoprecipitationen_US
dc.subjectTCDD-inducible poly-ADP-ribose polymerase (TIPARP)en_US
dc.subjectProtein purificationen_US
dc.subjectBiotinylated-NAD+en_US
dc.subject32P-NAD+en_US
dc.titleMethods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activityen_US
dc.status.refereedYesen_US
dc.date.Accepted2018
dc.date.application2018-08-11
dc.typeBook chapteren_US
dc.type.versionNo full-text in the repositoryen_US
refterms.dateFOA2021-02-10T15:05:22Z
dc.openaccess.statusNot Open Accessen_US


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