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dc.date.accessioned2021-02-10T15:04:22Z
dc.date.available2021-02-10T15:04:22Z
dc.date.issued2018
dc.identifier.citationHutin D, Grimaldi G and Matthews J (2018) Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity. In: Chang P (ed) ADP-ribosylation and NAD+ utilizing enzymes. Methods in Molecular Biology: 1813. Humana: New York.
dc.identifier.urihttp://hdl.handle.net/10454/18336
dc.descriptionNo
dc.description.abstractTCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.
dc.language.isoen
dc.subjectImmunoprecipitation
dc.subjectTCDD-inducible poly-ADP-ribose polymerase (TIPARP)
dc.subjectProtein purification
dc.subjectBiotinylated-NAD+
dc.subject32P-NAD+
dc.titleMethods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity
dc.status.refereedYes
dc.date.Accepted2018
dc.date.application2018-08-11
dc.typeBook chapter
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1007/978-1-4939-8588-3_8
refterms.dateFOA2021-02-10T15:05:22Z
dc.openaccess.statusclosedAccess


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