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    Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity

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    Publication date
    2018
    Author
    Hutin, D.
    Grimaldi, Giulia
    Matthews, J.
    Keyword
    Immunoprecipitation
    TCDD-inducible poly-ADP-ribose polymerase (TIPARP)
    Protein purification
    Biotinylated-NAD+
    32P-NAD+
    Peer-Reviewed
    Yes
    Open Access status
    Not Open Access
    
    Metadata
    Show full item record
    Abstract
    TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP- ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.
    URI
    http://hdl.handle.net/10454/18336
    Version
    No full-text in the repository
    Citation
    Hutin D, Grimaldi G and Matthews J (2018) Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity. In: Chang P (ed) ADP-ribosylation and NAD+ utilizing enzymes. Methods in Molecular Biology: 1813. Humana: New York.
    Link to publisher’s version
    https://doi.org/10.1007/978-1-4939-8588-3_8
    Type
    Book chapter
    Collections
    Life Sciences Publications

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