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dc.contributor.authorCheloni, Riccardo
dc.contributor.authorDenniss, Jonathan
dc.date.accessioned2020-11-06T13:51:40Z
dc.date.accessioned2020-11-20T14:15:40Z
dc.date.available2020-11-06T13:51:40Z
dc.date.available2020-11-20T14:15:40Z
dc.date.issued2021-1
dc.identifier.citationCheloni R and Denniss J (2020) Depth-resolved variations in visibility of retinal nerve fibre bundles across the retina in enface OCT images of healthy eyes. Ophthalmic and Physiological Optics. 41(1): 179-191.en_US
dc.identifier.urihttp://hdl.handle.net/10454/18175
dc.descriptionYesen_US
dc.description.abstractRecent developments in optical coherence tomography (OCT) technology enable direct enface visualisation of retinal nerve fibre bundle (RNFB) loss in glaucoma. However, the optimum depth at which to visualise RNFBs across the retina is unknown. We aimed to evaluate the range of depths and optimum depth at which RNFBs can be visualised across the retina in healthy eyes. The central ± 25° retina of 10 healthy eyes from 10 people aged 57–75 years (median 68.5 years) were imaged with spectral domain OCT. Slab images of maximum axial resolution (4 μm) containing depth‐resolved attenuation coefficients were extracted from 0 to 193.5 μm below the inner limiting membrane (ILM). Bundle visibility within 10 regions of a superimposed grid was assessed subjectively by trained optometrists (n = 8), according to written instructions. Anterior and posterior limits of RNFB visibility and depth of best visibility were identified for each grid sector. Effects of retinal location and individual eye on RNFB visibility were explored using linear mixed modelling with likelihood ratio tests. Intraclass correlation coefficient (ICC) was used to measure overall agreement and repeatability of grading. Spearman’s correlation was used to measure correlation between depth range of visible RNFBs and retinal nerve fibre layer thickness (RNFLT). Retinal location and individual eye affected anterior limit of visibility (χ2(9) = 58.6 and 60.5, both p < 0.0001), but none of the differences exceeded instrument resolution, making anterior limit consistent across the retina and different eyes. Greater differences were observed in the posterior limit of visibility across retinal areas (χ2(9) = 1671.1, p < 0.0001) and different eyes (χ2(9) = 88.7, p < 0.0001). Optimal depth for visualisation of RNFBs was around 20 µm below the ILM in most regions. It varied slightly with retinal location (χ2(9) = 58.8, p < 0.0001), but it was not affected by individual eye (χ2(9) = 10.7, p = 0.29). RNFB visibility showed good agreement between graders (ICC 0.89, 95%CI 0.87–0.91), and excellent repeatability (ICC 0.96–0.99). Depth range of visible RNFBs was highly correlated with RNFLT (ρ = 0.9, 95%CI: 0.86–0.95). The range of depths with visible RNFBs varies markedly across the healthy retina, consistently with RNFLT. To extract all RNFB information consistently across the retina, slab properties should account for differences across retinal locations and between individual eyes.en_US
dc.description.sponsorshipThis work was supported by a College of Optometrists Research Fellowship (JD).en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1111/opo.12756en_US
dc.rights© 2020 Wiley This is the peer reviewed version of the following article: Cheloni R and Denniss J (2020) Depth-resolved variations in visibility of retinal nerve fibre bundles across the retina in enface OCT images of healthy eyes. Ophthalmic and Physiological Optics. 41(1): 179-191, which has been published in final form at https://doi.org/10.1111/opo.12756 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en_US
dc.subjectGlaucomaen_US
dc.subjectOptical coherence tomographyen_US
dc.subjectOCTen_US
dc.subjectEnface imagingen_US
dc.subjectRetinal nerve fibre layeren_US
dc.subjectRetinal nerve fibre bundlesen_US
dc.titleDepth-resolved variations in visibility of retinal nerve fibre bundles across the retina in enface OCT images of healthy eyes.en_US
dc.status.refereedYesen_US
dc.date.Accepted2020-09-17
dc.date.application2020-11-04
dc.typeArticleen_US
dc.date.EndofEmbargo2021-11-05
dc.type.versionAccepted manuscripten_US
dc.description.publicnotesThe full-text of this article will be released for public view at the end of the publisher embargo on 5th Nov 2021.en_US
dc.date.updated2020-11-06T13:51:51Z
refterms.dateFOA2020-11-20T14:16:53Z


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