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    The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration

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    Publication date
    2020-07-07
    Author
    Whitelaw, J.A.
    Swaminathan, Karthic
    Kage, F.
    Machesky, L.M.
    Keyword
    Actin cytoskeleton
    WAVE complex
    Stress fibers
    Focal adhesions
    Migration
    Rights
    (c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (https://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    
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    Abstract
    Cells migrating over 2D substrates are required to polymerise actin at the leading edge to form lamellipodia protrusions and nascent adhesions to anchor the protrusion to the substrate. The major actin nucleator in lamellipodia formation is the Arp2/3 complex, which is activated by the WAVE regulatory complex (WRC). Using inducible Nckap1 floxed mouse embryonic fibroblasts (MEFs), we confirm that the WRC is required for lamellipodia formation, and importantly, for generating the retrograde flow of actin from the leading cell edge. The loss of NCKAP1 also affects cell spreading and focal adhesion dynamics. In the absence of lamellipodium, cells can become elongated and move with a single thin pseudopod, which appears devoid of N-WASP. This phenotype was more prevalent on collagen than fibronectin, where we observed an increase in migratory speed. Thus, 2D cell migration on collagen is less dependent on branched actin.
    URI
    http://hdl.handle.net/10454/17966
    Version
    Published version
    Citation
    Whitelaw JA, Swaminathan K, Kage F et al (2020) The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Cells. 9(7): 1635.
    Link to publisher’s version
    https://doi.org/10.3390/cells9071635
    Type
    Article
    Collections
    Life Sciences Publications

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