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dc.contributor.authorAkhtar, Shabana
dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorIsreb, Mohammad
dc.contributor.authorNewton, L.
dc.contributor.authorGopalan, Rajendran C.
dc.contributor.authorAnderson, Diana
dc.date.accessioned2020-06-15T16:36:17Z
dc.date.accessioned2020-07-06T14:33:09Z
dc.date.available2020-06-15T16:36:17Z
dc.date.available2020-07-06T14:33:09Z
dc.date.issued2020-07
dc.identifier.citationAkhtar S, Najafzadeh M, Isreb M et al (2020) Ex vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patients. Archives of Toxicology. 94(7): 2349-2357.en_US
dc.identifier.urihttp://hdl.handle.net/10454/17904
dc.descriptionYesen_US
dc.description.abstract2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) is a central dietary mutagen, produced when proteinaceous food is heated at very high temperatures potentially causing DNA strand breaks. This study investigates the protective potential of a well-researched flavonoid, myricetin in its bulk and nano-forms against oxidative stress induced ex vivo/in vitro by PhIP in lymphocytes from pre-cancerous monoclonal gammopathy of undetermined significance (MGUS) patients and those from healthy individuals. The results from the Comet assay revealed that in the presence of myricetin bulk (10 µM) and myricetin nano (20 µM), the DNA damage caused by a high dose of PhIP (100 µM) was significantly (P < 0.001) reduced in both groups. However, nano has shown better protection in lymphocytes from pre-cancerous patients. Consistent results were obtained from the micronucleus assay where micronuclei frequency in binucleated cells significantly decreased upon supplementing PhIP with myricetin bulk (P < 0.01) and myricetin nano (P < 0.001), compared to the PhIP treatment alone. To briefly determine the cellular pathways involved in the protective role of myricetin against PhIP, we studied gene expression of P53 and ATR kinase (ATM- and Rad3-related), using the real-time PCR technique.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://doi.org/10.1007/s00204-020-02754-xen_US
dc.rights(c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY licence (http://creativecommons.org/licenses/by/4.0/)en_US
dc.subjectATRen_US
dc.subjectBulk and nano formsen_US
dc.subjectLymphocytesen_US
dc.subjectMyricetinen_US
dc.subjectP53en_US
dc.subjectPhIPen_US
dc.subjectPre-cancerous patientsen_US
dc.titleEx vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patientsen_US
dc.status.refereedYesen_US
dc.date.Accepted2020-04-16
dc.date.application2020-04-27
dc.typeArticleen_US
dc.type.versionAccepted manuscripten_US
dc.date.updated2020-06-15T15:36:24Z
refterms.dateFOA2020-07-06T14:33:29Z


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