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    Ex vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patients

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    Publication date
    2020-07
    Author
    Akhtar, Shabana
    Najafzadeh, Mojgan
    Isreb, Mohammad
    Newton, L.
    Gopalan, Rajendran C.
    Anderson, Diana
    Keyword
    ATR
    Bulk and nano forms
    Lymphocytes
    Myricetin
    P53
    PhIP
    Pre-cancerous patients
    Rights
    (c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY licence (http://creativecommons.org/licenses/by/4.0/)
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) is a central dietary mutagen, produced when proteinaceous food is heated at very high temperatures potentially causing DNA strand breaks. This study investigates the protective potential of a well-researched flavonoid, myricetin in its bulk and nano-forms against oxidative stress induced ex vivo/in vitro by PhIP in lymphocytes from pre-cancerous monoclonal gammopathy of undetermined significance (MGUS) patients and those from healthy individuals. The results from the Comet assay revealed that in the presence of myricetin bulk (10 µM) and myricetin nano (20 µM), the DNA damage caused by a high dose of PhIP (100 µM) was significantly (P < 0.001) reduced in both groups. However, nano has shown better protection in lymphocytes from pre-cancerous patients. Consistent results were obtained from the micronucleus assay where micronuclei frequency in binucleated cells significantly decreased upon supplementing PhIP with myricetin bulk (P < 0.01) and myricetin nano (P < 0.001), compared to the PhIP treatment alone. To briefly determine the cellular pathways involved in the protective role of myricetin against PhIP, we studied gene expression of P53 and ATR kinase (ATM- and Rad3-related), using the real-time PCR technique.
    URI
    http://hdl.handle.net/10454/17904
    Version
    Accepted manuscript
    Citation
    Akhtar S, Najafzadeh M, Isreb M et al (2020) Ex vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patients. Archives of Toxicology. 94(7): 2349-2357.
    Link to publisher’s version
    https://doi.org/10.1007/s00204-020-02754-x
    Type
    Article
    Collections
    Life Sciences Publications

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